Patterns of in Vitro Labeling of Bone Marrow Lymphocytes and Antibody-producing Cells with Tritiated Thymidine and Deoxycytidine

• OSOGOE BUNSUKE

  Department of Anatomy, Kawasaki Medical School

• OKADA TERUHIKO

  Department of Anatomy, Kyoto University Faculty of Medicine

書誌事項

タイトル別名

• Patterns of <I>in Vitro</I> Labeling of Bone Marrow Lymphocytes and Antibody-producing Cells with Tritiated Thymidine and Deoxycytidine
• Difference between the Two Cell Types

抄録

Previous studies have revealed that among lymphocytes, two different types can be distinguished based on their patterns of labeling with ³H-thymidine (³HdThd)and/or ³H-deoxycytidine (³HdCyd). Thymic lymphocytes and germinal-center cells are to a large extent capables of utilizing deoxycytidine (dCyd) for the formation of DNAthymine. However, in such cells as pyroninophilic blast cells (or immunoblasts) and plasma cells, the capacity for utilizing dCyd is very limited. In the latter cells, the labeling intensity with ³HdThd is far stronger than that with ³HdCyd, but this is not the case for the former cells.<BR>In the present study, investigations were made of the patterns of in vitro labeling with ³HdThd and/or ³HdCyd of bone marrow lymphocytes of the guinea-pig and hemolytic plaque-forming cells (PFC) of the spleen of mice which had previously been stimulated with sheep red blood cells. The ability of these cells to utilize dCyd for the synthesis of DNA-thymine was also assessed. It was found that the bone marrow lymphocytes were as heavily labeled with ³HdThd as they were with ³HdCyd, but the antibodyproducing PFC showed far more intensive labeling with ³HdThd than with ³HdCyd. It was also found that the bone marrow lymphocytes had a strong capacity for utilizing dCyd in DNA-thmine formation, whereas such a capacity was very limited in the antibodyproducing PFC. These results indicate that, as regards patterns of labeling with radioactive deoxyribonucleosides, bone marrow lymphocytes are identical with thymic lymphocytes and germinal-center cells, but antibody-producing PFC are similar to pyroninophilic blast cells (or immunoblasts) and plasma cells. The significance of these findings is discussed in relation to the differentiation of lymphocytes and the pathways of DNA synthesis.<BR>It is concluded that, during the process of differentiation of bone marrow lymphocytes into antibody-producing cell, the pathway of dCyd utilization for DNA-thymine formation is partially or completely blocked.

収録刊行物

• Okajimas Folia Anatomica Japonica

  Okajimas Folia Anatomica Japonica 57 (2-3), 89-96, 1980

  Editorial Board of Okajimas Folia Anatomica Japonica

キーワード

• Bone marrow lymphocytes
• Antibody-producing cells
• <SUP>3</SUP>H-thymidine
• 8Hdeoxycytidine

詳細情報

• CRID: 1390282680437986048
• NII論文ID: 130004650068
• DOI: 10.2535/ofaj1936.57.2-3_89
• COI: 1:STN:280:DyaL3M7nsVSntA%3D%3D
• ISSN: 0030154X
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可