Measurement of bile Endothelin (ET) -1 using an enzyme immunoassay (EIA) and a chemiluminescent enzyme immunoassay (CLEIA)

• OKADA Toshihiro

  First Department of Surgery, Hyogo College of Medicine (Nishinomiya)

• YAMANAKA Naoki

  First Department of Surgery, Hyogo College of Medicine (Nishinomiya)

• TANAKA Tuneo

  First Department of Surgery, Hyogo College of Medicine (Nishinomiya)

• TANAKA Wataru

  First Department of Surgery, Hyogo College of Medicine (Nishinomiya)

• YASUI Chiaki

  First Department of Surgery, Hyogo College of Medicine (Nishinomiya)

• YAMANAKA Junichi

  First Department of Surgery, Hyogo College of Medicine (Nishinomiya)

• ANDOH Tatsuya

  First Department of Surgery, Hyogo College of Medicine (Nishinomiya)

• KURODA Nobukazu

  First Department of Surgery, Hyogo College of Medicine (Nishinomiya)

• KOH Motohiro

  First Department of Surgery, Hyogo College of Medicine (Nishinomiya)

• OKAMOTO Eizo

  First Department of Surgery, Hyogo College of Medicine (Nishinomiya)

Bibliographic Information

Other Title

• 胆汁中エンドセリン-1測定法
• EIA法およびCLEIA法を中心に

Abstract

Recent data suggested that ET is secreted not only by vascular endothelial cell but also by bile duct epithelial cell. However, there are conflicting results regarding to the detection of ET in the bile. This inconsistence may be caused by the methodology of measurement. In this study, we investigated whether or not ET-1 is detectable in human bile by using conventional, modified EIA and CLEIA. ET-1 was detected using conventional EIA only in the white bile with minimal bilirubin and bile acid, not in the common bile with a plenty of bilirubin and bile acid. In the process of the conventional preparation of bile samples, bilirubin and bile acid have, competitive inhibition with ET-1, precluding the abstraction of ET from the bile samples. With our technical modification of the preparation, a component of bilirubin and bile acid was separated and removed from the bile samples using chloroform and ion exchange resin (Amberlite XAD-2). This modified EIA generated significantly better recovery rate of ET-1, up to 45±9% in the choledocal bile. On the other hand, CLEIA, utilizing chemiluminescent activity of horseradish peroxidase, enabled a specific, sensitive and precise quantitive determination of ET-1 without such a preparation of the bile samples, as described above. By this assay system, the mean recovery rate of 69±21% for ET-1 was obtained in the choledochal bile. This study demonstrated the presence of ET-1 in the bile.

Journal

• Tando

  Tando 12 (3), 251-257, 1998

  Japan Biliary Association

Keywords

• Endothelin-1
• enzyme immunoassay(EIA)
• chemiluminescent enzyme immunoassay(CLEIA)

Details

• CRID: 1390001205466975232
• NII Article ID: 130004675580
• DOI: 10.11210/tando1987.12.3_251
• ISSN: 09140077
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed