Preparation and evaluation of highly dispersible and Ca²⁺ level responsive PEGylated calcium phosphate nanoparticle with PEG-<i>block</i>-poly(4-vinylbenzylphosphonate) for the application of drug carrier

• Asai Kei

  Department of Materials Science, Graduate School of Pure and Applied Sciences, University of Tsukuba

• Kanayama Naoki

  Department of Materials Science, Graduate School of Pure and Applied Sciences, University of Tsukuba

• Nagasaki Yukio

  Department of Materials Science, Graduate School of Pure and Applied Sciences, University of Tsukuba Master's School of Medicinal Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba International Center for Materials Nanoarchitectonics Satellite (MANA), National Institute for Materials Science (NIMS) and University of Tsukuba

抄録

PEGylated calcium phosphate nanoparticles (CaPNPs) were prepared through the simple mixing of calcium and phosphate solutions in the presence of a PEG-block-poly(4-vinylbenzylphosphonate) (PEG-b-PVBP). The PEG-b-PVBP possesses the phosphonate side chains and the hydrophobic polystyrene segment which enhance the stability of the PEG brush layer on the CaPNP surface. The size of CaPNPs was controlled within the range of several tens of nanometer depending on the [PVBP unit]/[phosphate ion] feed-molar ratio upon the CaP coprecipitation reaction. The obtained CaPNPs showed excellent colloidal stability and it was sustained even after the lyophilization. In vitro cell cytotoxicity assay suggested that the CaPNPs do not induce serious cytotoxicity in the murine colon adenocarcinoma 26 (C-26) cells. Light scattering study revealed that the CaPNPs can be dissolved into the low Ca²⁺ condition ([Ca²⁺] = 0.1 mM). Since intracellular Ca²⁺ concentration is four orders of magnitude lower than the extracellular one, the Ca²⁺ concentration responsive dissolution behavior of CaPNPs is promising for the intracellular delivery of therapeutic materials.

収録刊行物

• Transactions of the Materials Research Society of Japan

  Transactions of the Materials Research Society of Japan 37 (3), 337-340, 2012

  一般社団法人 日本MRS

キーワード

• PEG-based block copolymer
• surface modification
• calcium phosphate nanoparticle
• calcium responsive

詳細情報

• CRID: 1390001205513510400
• NII論文ID: 130004676398
• DOI: 10.14723/tmrsj.37.337
• COI: 1:CAS:528:DC%2BC38XhslSksrvN
• ISSN: 21881650
• ISSN: 13823469
• Web Site: https://www.jstage.jst.go.jp/article/tmrsj/37/3/37_337/_pdf
• 本文言語コード: en
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