Correlations among antral follicular echotexture, apoptosis and expression of key steroidogenic enzymes in sheep

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  • VANDUZER Taylor
    Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph ON, N1G 2W1 Canada
  • DUGGAVATHI Raj
    Department of Animal Science, McGill University, Ste-Anne-de-Bellevue QC, H9X 3V9 Canada
  • MURAWSKI Maciej
    Department of Swine and Small Ruminant Breeding, University of Agriculture in Kraków, 30-274 Kraków, Poland
  • ZIEBA Dorota A.
    Department of Swine and Small Ruminant Breeding, University of Agriculture in Kraków, 30-274 Kraków, Poland
  • SROKA Patrycja
    Department of Swine and Small Ruminant Breeding, University of Agriculture in Kraków, 30-274 Kraków, Poland
  • BARTLEWSKI Pawel M.
    Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph ON, N1G 2W1 Canada

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Nineteen cycling ewes underwent transrectal ultrasonography of ovaries followed by ovariectomies during the growth phase of the first follicular wave of the interovulatory interval or the proestrus/estrus phase of the cycle. Quantitative ultrasonographic characteristics of the antrum and follicular wall in a total of forty-three ovine antral follicles were examined for correlations with the protein expression of three steroidogenic enzymes (cytochrome P450 17α-hydroxylase, CYP17; cytochrome P450 aromatase, CYP19; and 3β-hydroxysteroid dehydrogenase, 3β-HSD) determined by densitometric analysis of immunohistochemical slides, follicular dimensions, granulosa layer thickness and the percentage of apoptotic granulosa cells. Significant correlations were found between echotextural attributes of ovine antral follicles and the percentage of apoptotic granulosa cells, CYP17 expression (theca), CYP19 expression (granulosa) and 3β-HSD expression (theca cells). Computer-aided analyses of ultrasonographic images can be beneficial to the development of assisted reproductive technologies and diagnosis of hormonal imbalances without the need for ovarian biopsies or hormone assays.

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