The blood cell flow and the vascular responses in arterioles and capillaries after subarachnoid hemorrhage
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- Ishikawa Mami
- Department of Biochemistry, School of Medicine, Keio University Department of Neurosurgery, Jichi Medical University
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- Kajimura Mayumi
- Department of Biochemistry, School of Medicine, Keio University
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- Morikawa Takayuki
- Department of Biochemistry, School of Medicine, Keio University
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- Nakamura Tomomi
- Department of Biochemistry, School of Medicine, Keio University
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- Tanaka Yuichi
- Department of Neurosurgery, Jichi Medical University
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- Watanabe Eiju
- Department of Neurosurgery, Jichi Medical University
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- Suematsu Makoto
- Department of Biochemistry, School of Medicine, Keio University
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Purpose: Immediately after subarachnoid hemorrhage (SAH), brain injury begins and determines the acute phase mortality and the long-term prognosis, but its mechanism is not well understood. When SAH at the skull base induces platelet-leukocyte-endothelial cell interactions in venules, the cerebral blood flow is kept well at the cerebral surface1). We investigated cerebral microcirculation through a mouse cranial window using two-photon laser scanning microscopy at a depth of about 100µm2,3), after SAH was induced at the skull base.<br>Methods: Tracheotomy was performed and femoral artery was cannulated in mice (FVB/N-Tg (GFAP GFP) 14Mes/j). Q-dot 655 nanocrystal (Q21021MP; Invitrogen) or rhodamine-6G was injected from the cannulated femoral vein, after a craniotomy at the parietal bone without cutting dura matter. SAH was induced at a prone position by using the endovascular perforation model4). Immediately and one hour after SAH, blood cell velocities were measured with a line scan method in precapillary and capillary using two-photon laser scanning microscopy.<br>Results: A penetrating arteriole branched into a precapillary arteriole at the depth of 85.9 +/- 21.0µm (n=7). Arterioles dilated immediately after SAH and then gradually constricted (n=5/7) and the blood flow disappeared immediately after SAH in the others (n=2/7). The blood cell velocity of the precapillary arteriole decreased from 10.7 +/- 3.0 mm/s before SAH to 0.9 +/- 0.4 mm/s after SAH. The capillary-velocities of blood cells (red blood cells, platelets and leukocytes) also decreased, and rolling and adherent leukocytes prevented blood cells from flowing in capillaries. <br>Conclusion: The cerebral blood flow decreases in arterioles and capillaries, when the SAH is induced.
収録刊行物
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- Microvascular Reviews and Communications
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Microvascular Reviews and Communications 7 (1), 35-35, 2014
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詳細情報 詳細情報について
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- CRID
- 1390282680232230912
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- NII論文ID
- 130004678473
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- ISSN
- 18805906
- 21881707
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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