An Organophosphate Sensor Based on Photo-crosslinked Hydrogel-entrapped E. coli

DOI PDF Web Site Web Site Web Site ほか1件をすべて表示 一部だけ表示 参考文献27件

書誌事項

タイトル別名
  • An Organophosphate Sensor Based on Photo-crosslinked Hydrogel-entrapped <i>E. coli</i>

この論文をさがす

抄録

This paper describes a whole cell sensor using E. coli entrapped within photocrosslinked hydrogel beads. Hydrogel beads containing organophosphorus hydrolase (OPH)-expressed E. coli were prepared by adding a hydrogel precursor solution containing the E. coli to an oil phase using a precision syringe pump, forming droplets, and photopolymerizing them. The beads showed good monodispersity with an average size of 1.2 mm. We detected organophosphates (OPs) using the beads. The detection relied on a pH-sensitive fluorescence dye that responds to protons produced from the intracellular OPH reaction with the OPs. This sensor could detect up to 80 μM of paraoxon with a detection limit of 3 μM. The enzyme activity of E. coli entrapped within the hydrogel beads showed stable enzyme activity for at least two weeks. This whole cell sensor will be implemented in a microfluidic system by directly photopolymerizing the hydrogel precursor solution within microfluidic channels.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 30 (9), 937-942, 2014

    社団法人 日本分析化学会

参考文献 (27)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ