mRNA Expression and Localization of LPS-induced β-defensin Isoforms in Rat Salivary Glands

DOI PubMed 被引用文献1件 参考文献19件
  • Shinomiya Takashi
    Department of Pharmacology, Tokyo Dental College
  • Kawaguchi Mitsuru
    Department of Pharmacology, Tokyo Dental College
  • Okubo Migiwa
    Department of Pharmacology, Tokyo Dental College
  • Kosuge Yasuhiro
    Department of Pharmacology, Tokyo Dental College Laboratory of Pharmacology, School of Pharmacy, Nihon University
  • Yoshikawa Masanobu
    Department of Pharmacology, Tokyo Dental College Department of Clinical Pharmacology, Tokai University School of Medicine

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β-defensins are small, cationic peptides with broad-spectrum antimicrobial activity that are produced by mucosal epithelia. However, little is known about the expression of β-defensins in the major salivary glands. The purpose of this study was to characterize expression of rat β-defensin-1 (RBD-1) and -2 (RBD-2) mRNA within the major salivary glands together with the effect of injection of intraductal lipopolysaccharide (LPS) on that expression. β-defensin mRNA expression was quantitated by RT-PCR in salivary gland tissues and salivary acinar and striated duct cells collected by laser captured microdissection. RBD-1 and -2 were expressed in the parotid gland, the submandibular gland, and the sublingual gland. β-defensins were expressed in both the acinar and striated duct cells of the major salivary glands. Intraductal injection of LPS increased expression of RBD-1 and -2 mRNA, which peaked at 12 hrs. These results suggest that salivary cells (acinar and striated duct cells) have the potential to produce β-defensins.

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