Serum miRNA profiling identifies miR-150/30a as potential biomarker for workers with damaged nerve fibers from carbon disulfide
-
- GUO Li
- Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, China
-
- LUO Chen
- Department of Occupational Medicine and Environmental Health, School of Public Health, Nanjing Medical University, China
-
- FAN Jingjing
- Department of Occupational Medicine and Environmental Health, School of Public Health, Nanjing Medical University, China
-
- HOU Zhiguo
- Department of Occupational Medicine and Environmental Health, School of Public Health, Nanjing Medical University, China
-
- JI Xiaoming
- Department of Occupational Medicine and Environmental Health, School of Public Health, Nanjing Medical University, China
-
- CHEN Feng
- Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, China
-
- ZHU Baoli
- Center for Disease Control and Prevention of Jiangsu Province, China
-
- NI Chunhui
- Department of Occupational Medicine and Environmental Health, School of Public Health, Nanjing Medical University, China
この論文をさがす
抄録
As crucial small regulatory molecules, serum microRNAs (miRNAs) have been widely identified as potential noninvasive biomarkers. To survey and identify serum miRNAs associated with workers who had experienced injury to their nerve system from carbon disulfide (CS2), we profiled abnormally expressed miRNAs using the microarray technique and further performed qRT-PCR validation in case and control samples (n=20). Microarray profiling in pooled RNA samples showed that many miRNAs in workers exposed to CS2 were aberrantly expressed. Based on control samples exposed to CS2, a great amount of abnormal miRNAs, including some miRNA gene clusters and families, were obtained from microarray datasets. Most of deregulated miRNAs were up-regulated, and almost all miRNAs showed consistent expression patterns between workers with different numbers of damaged nerve fibers. Functional enrichment analysis suggested that these abnormal miRNAs showed versatile roles by contributing to multiple biological processes. Some aberrantly expressed miRNAs were characterized as miRNA gene clusters or families, and they always showed consistent expression patterns. miR-150 and miR-30a were selected to be further validated by qRT-PCR as up-regulated species, and they could discern case samples from control samples. miR-150 and miR-30a may be potential noninvasive biomarkers for a damaged nervous system.
収録刊行物
-
- Industrial health
-
Industrial health 53 (1), 38-47, 2015
独立行政法人 労働者健康安全機構 労働安全衛生総合研究所
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679258127872
-
- NII論文ID
- 130004687758
- 40020365529
-
- NII書誌ID
- AA00672955
-
- ISSN
- 18808026
- 00198366
-
- NDL書誌ID
- 026150785
-
- PubMed
- 25224332
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可