A Spectrophotometric Assay for Choline in Enteral Nutrition

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  • 経腸栄養剤中のコリンの吸光光度定量
  • ケイ チョウ エイヨウザイ チュウ ノ コリン ノ キュウコウ コウド テイリョウ

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Abstract

A spectrophotometric method was developed and validated for choline in enteral nutrition. The method involves an enzymatic conversion of choline by choline oxidase to hydrogen peroxide, which reacts with 3-(N-ethyl-3-methylanilino)-2-hydroxypropanesulfonic acid (TOOS) and 4-aminoantipyrine in the presence of peroxidase to form a purple colored product with the optimal absorption at 554 nm. The test conditions were optimized for avoiding interference by any active ingredient other than choline in a commercial drug product (ELENTAL®). This method was validated for the determination of choline bitartrate in the ELENTAL®, and demonstrated good specificity, linearity, accuracy and precision. The proposed method was successfully applied to assays of choline in commercial drug products (ELENTAL® and HEPAN ED®). The proposed method is simple, specific and useful for the determination of choline in enteral nutrition and supplements.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 63 (9), 743-749, 2014

    The Japan Society for Analytical Chemistry

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