Therapeutic effect of Xue Niao An on glyoxylate-induced calcium oxalate crystal deposition based on urinary metabonomics approach
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- Peng Zhongjiang
- Department of Nephrology, Changhai Hospital, Second Military Medical University
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- Chen Wei
- Department of Nephrology, Changhai Hospital, Second Military Medical University
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- Gao Songyan
- School of Pharmacy, Second Military Medical University
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- Su Li
- School of Pharmacy, Second Military Medical University
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- Li Na
- School of Pharmacy, Second Military Medical University
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- Wang Li
- Department of Nephrology, Changhai Hospital, Second Military Medical University
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- Lou Ziyang
- School of Pharmacy, Second Military Medical University
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- Dong Xin
- School of Pharmacy, Second Military Medical University
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- Guo Zhiyong
- Department of Nephrology, Changhai Hospital, Second Military Medical University
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抄録
The anti-nephrolithiasis effect of Xue Niao An (XNA) capsules is explored by analyzing urine metabolic profiles in mouse models, with ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). An animal model of calcium oxalate crystal renal deposition was established in mice by intra-abdominal injection of glyoxylate. Then, treatment with XNA by intra-gastric administration was performed. At the end of the study, calcium deposition in kidney was measured by Von Kossa staining under light microscopy, and the Von Kossa staining changes showed that XNA significantly alleviated the calcium oxalate crystal deposition. Meanwhile, urine samples for fifteen metabolites, including amino acids and fatty acids, with significant differences were detected in the calcium oxalate group, while XNA treatment attenuated metabolic imbalances. Our study indicated that the metabonomic strategy provided comprehensive insight on the metabolic response to XNA treatment of rodent renal calcium oxalate deposition.
収録刊行物
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- Ｊｏｕｒｎａｌ ｏｆ Ｃｌｉｎｉｃａｌ Ｂｉｏｃｈｅｍｉｓｔｒｙ ａｎｄ Ｎｕｔｒｉｔｉｏｎ
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Ｊｏｕｒｎａｌ ｏｆ Ｃｌｉｎｉｃａｌ Ｂｉｏｃｈｅｍｉｓｔｒｙ ａｎｄ Ｎｕｔｒｉｔｉｏｎ 55 (3), 184-190, 2014
一般社団法人 日本酸化ストレス学会