Characterization of Enamelysin (MMP-20) Expressed in the Porcine Odontoblast

DOI
  • UCHIDA Takashi
    Department of Oral Biology, Division of Molecular Medical Science, Hiroshima University Graduate School of Biomedical Science
  • JAHAN Ishrat
    Department of Oral Biology, Division of Molecular Medical Science, Hiroshima University Graduate School of Biomedical Science
  • KARUKAYA-KAKUO Emi
    Department of Oral Biology, Division of Molecular Medical Science, Hiroshima University Graduate School of Biomedical Science
  • MURAKAMI-KUBO Chikage
    Department of Oral Biology, Division of Molecular Medical Science, Hiroshima University Graduate School of Biomedical Science
  • WAKIDA Kazuyoshi
    Department of Oral Biology, Division of Molecular Medical Science, Hiroshima University Graduate School of Biomedical Science
  • HAYASHIDA Koichi
    Department of Oral Biology, Division of Molecular Medical Science, Hiroshima University Graduate School of Biomedical Science
  • HIYAMA Shinji
    Department of Oral Biology, Division of Molecular Medical Science, Hiroshima University Graduate School of Biomedical Science
  • YAMANISH Emiko
    Department of Oral Biology, Division of Molecular Medical Science, Hiroshima University Graduate School of Biomedical Science
  • SATODA Takahiro
    Department of Oral Biology, Division of Molecular Medical Science, Hiroshima University Graduate School of Biomedical Science

書誌事項

タイトル別名
  • <b>Characterization of Enamelysin (MMP-20) Expressed in the Porcine </b><b>Odontoblast </b>

この論文をさがす

抄録

Enamelysin (MMP-20) is a matrix metalloproteinase isolated from the cDNA library of the enamel organ. Although enamelysin is also expressed in the tooth pulp, especially in the odontoblast, the functional significance of enamelysin protein in dentinogenesis has not been elucidated. Thus, the present study was designed to clarify the characteristics of enamelysin in the pulp from the porcine deciduous molar tooth germ and developing teeth. Western blot analysis of proteins extracted from the pulp of the tooth germ revealed that immunoreactive enamelysin occurred as 54 kDa and 51 kDa proteins. None of the protein bands lower than 51 kDa was stained. Light and electron microscopic immunohistochemistry of tooth germs and erupting teeth demonstrated positive reactions over the cytoplasmic matrix of odontoblast cell bodies and processes (dentinal fibers). The secretoty machinery of the odontoblast was shown to be devoid of immunoreactivity. Intensity of the immunoreaction in the odontoblast was variable but essentially dependent upon the position and differentiation stage of the odontoblast. The highest immunoreactivity was found in some odontoblasts located at the cuspal region of the tooth germ. During tooth eruption, cell bodies of odontoblasts in the crown showed faint or no immunoreactivity. No immunoreaction was detected in the odontoblast facing the root dentin, although dentinal fibers of the root dentin showed faint immunoreactivity. RT-PCR and cDNA sequence analysis failed to find any differences between enamelysin mRNA extracted from the enamel organ and that from the pulp. These results suggest that odontoblasts transiently express enamelysin during tooth formation and that the enamelysin in the odontoblast is not secreted from the cell.

収録刊行物

  • Biomedical Research

    Biomedical Research 24 (4), 205-216, 2003

    バイオメディカルリサーチプレス

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ