等時相線図による流体解析を用いた人工毛細血管網の可視化に関する研究

• 外處 侑

• 野口 展士

• 荒船 龍彦

• 幡多 徳彦

• 野中 一洋

• 本間 章彦

• 舟久保 昭夫

書誌事項

タイトル別名

• Study on the visualization of an artificial capillary network by using isochronal map

抄録

In the 3D cell culture, it is necessary to construct optimum capillaries, which supply oxygen and nutrients for the cells. As we already reported the blood capillaries had been found that formed 5-20μm length in 3D scaffolds in optimum flow rate culture condition. The purpose of this study is the visualizing culture medium flow in 3D scaffold to recognize the process of the capillary network constructions in 3D scaffold. A small vascular graft that created 0.4mm holes at 5mm intervals in wall was sandwiched between the fibrous scaffold sheets. The visualizing particles were flowed to scaffold. The culture medium flow were captured as visualizing particle flow using high speed video camera(Hurricane-40/Photon focus, 640x480pixels, 100fps). The captured flow images were processed as Isochronal Map in offline and were compared with cultured capillary image measured by phase contrast microscope.

収録刊行物

• 生体医工学

  生体医工学 52 (Supplement), O-241-O-241, 2014

  公益社団法人 日本生体医工学会

詳細情報

• CRID: 1390001205267539328
• NII論文ID: 130004695792
• DOI: 10.11239/jsmbe.52.o-241
• ISSN: 18814379
• ISSN: 1347443X
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可