Complement-dependent cytotoxic crossmatchおよびFlow cytometric crossmatchの結果の乖離についての検討―補体結合性HLA抗体の検出―

DOI 機関リポジトリ HANDLE 参考文献15件
  • 石塚 敏
    東京女子医科大学腎臓病総合医療センター移植免疫研究室
  • 安尾 美年子
    東京女子医科大学腎臓病総合医療センター移植免疫研究室
  • 石田 悠梨
    東京女子医科大学腎臓病総合医療センター移植免疫研究室
  • 二ツ山 和也
    東京女子医科大学腎臓病総合医療センター移植免疫研究室
  • 吉野 敏栄
    東京女子医科大学腎臓病総合医療センター移植免疫研究室
  • 岩藤 和広
    東京女子医科大学腎臓病総合医療センター腎臓外科
  • 中島 一朗
    東京女子医科大学腎臓病総合医療センター腎臓外科
  • 渕之上 昌平
    東京女子医科大学腎臓病総合医療センター腎臓外科

書誌事項

タイトル別名
  • Is the discrepancy between complement-dependent cytotoxic crossmatch and flow cytometric crossmatch due to the difference in complement-binding capacity of HLA antibodies?

この論文をさがす

抄録

【Objective】There may be a discrepancy in the results between the complement-dependent cytotoxic crossmatch test (CDC-XM) and the flow cytometry crossmatch test (FCXM) for the detection of HLA antibodies (HLA-Abs). We investigated the cause of this discrepancy using the solid-phase assay method with the LABScreen single antigen (LABScreen) and the LABScreen single C1q (C1qScreen). <br>【Methods】Twenty-one candidates for renal transplantation who visited our outpatient clinic in 2011 were HLA-Abs positive by FlowPRA. Their serums underwent the LABScreen test and the C1qScreen test. The specificity of the Abs in these serums was determined using LABScreen and lymphocyte cells (LCCs) of a third party that had the same antigen as the specified Abs that were selected. Then these LCCs underwent FCXM and CDC-XM. The results of the tests were compared with each other. Moreover, we studied the clinical outcomes of 9 recipients that had tested positive by FCXM and negative by CDC-XM preoperatively. We also studied 3 cases as references of poor graft that had tested negative by FCXM and CDC-XM and developed rejection.<br>【Results】HLA-Abs that were positive by FCXM using LCCs exhibited 26 kinds of classⅠ and Ⅱ antibodies, and the results of C1qScreen and CDC-XM showed significant correlation with them（p＜0.001）. Those of C1qScreen and LABScreen, however, showed no significant correlation. In 12 recipients who were all CDC-XM negative before transplantation, 9 became LABScreen positive; postoperatively, 4 recipients became C1qScreen positive. Graft loss and C1qScreen of chronic-AMR cases were all positive after transplantation.<br>【Conclusion】C1qScreen was not always positive, even when FCXM was positive and LABScreen detected a high quantity of HLA-Abs, but C1qScreen was almost always positive when CDC-XM was. That indicates that discrepancy in the results of CDC-XM and FCXM was caused to some extent by the dependency of HLA-Abs on complement. Furthermore, C1qScreen showed positive results in all chronic AMR and graft loss cases, suggesting that complement-dependent donor specific antibodies were partially involved in chronic-AMR and graft loss.

収録刊行物

  • 移植

    移植 48 (1), 033-041, 2013

    一般社団法人 日本移植学会

参考文献 (15)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ