Folliculostellate Cells Are Required for Laminin Release from Gonadotrophs in Rat Anterior Pituitary

• Tsukada Takehiro

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Fujiwara Ken

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Horiguchi Kotaro

  Laboratory of Anatomy and Cell Biology, Department of Health Sciences, Kyorin University

• Azuma Morio

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Ramadhani Dini

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Tofrizal Alimuddin

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Batchuluun Khongorzul

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Maliza Rita

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Syaidah Rahimi

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Kikuchi Motoshi

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine Laboratory of Natural History, Jichi Medical University School of Medicine

• Yashiro Takashi

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

抄録

The anterior pituitary gland is organized tissue comprising hormone-producing cells and folliculostellate (FS) cells. FS cells interconnect to form a meshwork, and their cytoplasmic processes are anchored by a basement membrane containing laminin. Recently, we developed a three-dimensional (3D) cell culture that reproduces this FS cell architecture. In this study of the novel function of FS cells, we used transgenic rats that express green fluorescent protein in FS cells for the 3D culture. Anterior pituitary cells were cultured with different proportions of FS cells (0%, 5%, 10%, and 20%). Anterior pituitary cells containing 5–20% FS cells formed round/oval cell aggregates, whereas amorphous cell aggregates were formed in the absence of FS cells. Interestingly, immunohistochemistry showed laminin-immunopositive cells instead of extracellular laminin deposition in FS cell-deficient cell aggregates. Double-immunostaining revealed that these laminin-immunopositive cells were gonadotrophs. Laminin mRNA expression did not differ in relation to the presence or absence of FS cells. When anterior pituitary cells with no FS cells were cultured with FS cell-conditioned medium, the proportion of laminin-immunopositive cells was lower than in control. These results suggest that a humoral factor from FS cells is required for laminin release from gonadotrophs.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 47 (5), 239-245, 2014

  日本組織細胞化学会

キーワード

• laminin
• anterior pituitary
• folliculostellate cell
• gonadotroph
• 3D cell culture

詳細情報

• CRID: 1390001204862502016
• NII論文ID: 130004700245
• DOI: 10.1267/ahc.14036
• ISSN: 13475800
• ISSN: 00445991
• Web Site: https://www.jstage.jst.go.jp/article/ahc/47/5/47_14036/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015382876
• 本文言語コード: en
• データソース種別:
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