Parasitology : Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa

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  • NGUYEN Thu-Thuy
    OIE Reference Laboratory for Surra, OIE Collaborating Centre, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
  • MOTSIRI Mono Sophie
    Parasitology Research Program, Department of Zoology and Entomology, University of the Free State–Qwaqwa Campus, Private Bag, X13, Phuthaditjhaba 9866, South Africa
  • TAIOE Moeti Oriel
    Parasitology Research Program, Department of Zoology and Entomology, University of the Free State–Qwaqwa Campus, Private Bag, X13, Phuthaditjhaba 9866, South Africa
  • MTSHALI Moses Sibusiso
    Parasitology Research Program, Department of Zoology and Entomology, University of the Free State–Qwaqwa Campus, Private Bag, X13, Phuthaditjhaba 9866, South Africa Veterinary Parasitology Unit, Research and Scientific Services Department, National Zoological Gardens of South Africa, Pretoria 0001, South Africa
  • GOTO Yasuyuki
    Laboratory of Molecular Immunology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
  • KAWAZU Shin-Ichiro
    OIE Reference Laboratory for Surra, OIE Collaborating Centre, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
  • THEKISOE Oriel Matlhahane Molifi
    Parasitology Research Program, Department of Zoology and Entomology, University of the Free State–Qwaqwa Campus, Private Bag, X13, Phuthaditjhaba 9866, South Africa
  • INOUE Noboru
    OIE Reference Laboratory for Surra, OIE Collaborating Centre, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

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  • Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa

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A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic test (ICT) were also used. Crude antigen ELISA, TeGM6-4r-ELISA and ICT detected 27.3%, 29% and 19.9% of trypanosome seropositive samples, respectively. Trypanosome infection prevalence in cattle and goats was 35.8–46.3% and 0–9.1%, respectively. Out of 9 sheep serum samples, 2–4 sera (22.2–44.4%) were positive. The detection performance of crude and recombinant antigen ELISAs was relatively similar (K=0.6–0.7); both are recommended for reference diagnosis and large scale epidemiological surveys. There is potential application for ICT in on-site diagnosis, but its sensitivity should be improved.

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