The Identification of Sex-Chromosomes and Karyological Analysis of Rice Frog, <i>Fejervarya limnocharis</i>(Anura, Ranidae) from Northeast Thailand

• Patawang Isara

  Genetics and Environmental Toxicology Research Group, Department of Biology, Faculty of Science, Khon Kaen University

• Tanomtong Alongklod

  Genetics and Environmental Toxicology Research Group, Department of Biology, Faculty of Science, Khon Kaen University

• Phimphan Sumalee

  Genetics and Environmental Toxicology Research Group, Department of Biology, Faculty of Science, Khon Kaen University

• Chuaynkern Yodchaiy

  Genetics and Environmental Toxicology Research Group, Department of Biology, Faculty of Science, Khon Kaen University

• Chuaynkern Chantip

  Genetics and Environmental Toxicology Research Group, Department of Biology, Faculty of Science, Khon Kaen University

• Phaengphairee Pheeraphong

  Department of Animal Science, Faculty of Agriculture, Khon Kaen University

• Khrueanet Wilailuk

  Faculty of Applied Science and Engineering, Nong Kai Campus, Khon Kaen University

• Nithikulworawong Nudtha

  Faculty of Applied Science and Engineering, Nong Kai Campus, Khon Kaen University

Abstract

Karyological analysis of the rice frog (Fejervarya limnocharis) from Northeast Thailand was conducted. Frog chromosome preparations were obtained by the squash technique from bone marrow and testis. Conventional staining and Ag-NOR banding techniques were applied to stain the chromosome with Giemsa's solution. The results showed that the number of diploid chromosome is 2n = 26, while the fundamental number (NF) is 52 in both males and females. The types of autosome were 4 large metacentric, 6 large submetacentric, 8 small metacentric, 4 small submetacentric and 2 small acrocentric chromosomes. The present study also revealed that the sex chromosome system of F. limnocharis was XY, in which the X and Y were classified as small metacentric and small submetacentric chromosomes, respectively. Nucleolar organizer regions (NORs) are located at the secondary constriction of the short arm near the centromere in submetacentric chromosome pair 6. We found that during metaphase I the homologous chromosomes showed synapsis, which can be defined as the 13 ring bivalents and 13 haploid chromosomes at metaphase II as diploid species. The karyotype formula is as follows:<br>2n (26) = L₄^m+L₆^sm+S₈^m+S₄^sm+S^a₂+sex-chromosomes (XY)

Journal

• CYTOLOGIA

  CYTOLOGIA 79 (2), 141-150, 2014

  Japan Mendel Society, International Society of Cytology

Keywords

• Rice frog
• <i>Fejervarya limnocharis</i>
• Chromosome
• Karyotype

Details

• CRID: 1390282679086502400
• NII Article ID: 130004701652
• DOI: 10.1508/cytologia.79.141
• ISSN: 13487019
• ISSN: 00114545
• Web Site: https://www.jstage.jst.go.jp/article/cytologia/79/2/79_141/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed