Qing Dai attenuates nonsteroidal anti-inflammatory drug-induced mitochondrial reactive oxygen species in gastrointestinal epithelial cells

• Saito Rie

  Faculty of Medicine, University of Tsukuba

• Tamura Masato

  Faculty of Medicine, University of Tsukuba

• Matsui Hirofumi

  Faculty of Medicine, University of Tsukuba

• Nagano Yumiko

  Faculty of Medicine, University of Tsukuba

• Suzuki Hideo

  Faculty of Medicine, University of Tsukuba

• Kaneko Tsuyoshi

  Faculty of Medicine, University of Tsukuba

• Mizokami Yuji

  Faculty of Medicine, University of Tsukuba

• Hyodo Ichinosuke

  Faculty of Medicine, University of Tsukuba

抄録

Treatments with nonsteroidal anti-inflammatory drugs (NSAIDs) have increased the number of patients with gastrointestinal complications. Qing Dai has been traditionally used in Chinese herbal medicine for various inflammatory diseases such as ulcerative colitis. We previously reported that Qing Dai suppressed inflammations by scavenging reactive oxygen species (ROS) in ulcerative colitis patients. Thus, Qing Dai can attenuate the production of ROS, which play an important role in NSAID-induced gastrointestinal injuries. In this study, we aimed to elucidate whether Qing Dai decreased mitochondrial ROS production in NSAID-treated gastrointestinal cells by examining cellular injury, mitochondrial membrane potentials, and ROS production with specific fluorescent indicators. We also performed electron paramagnetic resonance measurement in isolated mitochondria with a spin-trapping reagent (CYPMPO or DMPO). Treatments with indomethacin and aspirin induced cellular injury and mitochondrial impairment in the gastrointestinal cells. Under these conditions, mitochondrial alterations were observed on electron microscopy. Qing Dai prevented these complications by suppressing ROS production in gastrointestinal cells. These results indicate that Qing Dai attenuated the ROS production from the NSAID-induced mitochondrial alteration in the gastrointestinal epithelial cells. Qing Dai treatment may be considered effective for the prevention NSAID-induced gastrointestinal injury.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ 56 (1), 8-14, 2015

  一般社団法人　日本酸化ストレス学会

キーワード

• Qing Dai
• ROS
• NSAIDs
• gastrointestinal injury
• mitochondria

詳細情報

• CRID: 1390001204671986048
• NII論文ID: 130004704975
• DOI: 10.3164/jcbn.14-59
• ISSN: 18805086
• ISSN: 09120009
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可