マウスの膵島分離から臨床試験へ

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  • From mouse islet isolation to clinical islet transplantation trial in Japan

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My research started in 1979 to investigate counter-regulatory secretion and action of insulin and glucagon on glucose metabolism in pancreas auto/allografts using mongrel dogs. I then had the opportunity to study pancreas islet transplantation in 1984, when I worked as a research fellow with Prof. A. P. Monaco in New England Deaconess Hospital. The first success of establishment of islet isolation method in mouse facilitated research process on the studies of islet immunology and tolerance induction. Specific results are summarized as follows. 1) Stationary digestion of the pancreas following intraductal collagenase injection is a method to isolate reproducibly high yields of islets from rodent pancreata, 2)handpicked islets are less immunogenic compared with crude islets, 3)the number of transplanted islets is critical to induce long-term graft survival in nonimmunosuppressed recipients, 4) further reducing immunogenicity could be obtained by using multiple donors with diverse histocompatibilities, 5)antilymphocyte serum has unique and potent actions on inducing tolerance through manipulation of donor and recipient immune system, 6) immunogenicity of islet graft can be altered by gamma-irradiation. Even today these results are fresh in my memory. I always believe in the words that nature does not tell a lie just as an exquisite wine served together with a matching appetizer. I conclude my talk with special thanks to distinguished researchers who have been involved in ongoing government-supported clinical islet transplantation trials in Japan(UMIN000003977).

収録刊行物

  • Organ Biology

    Organ Biology 20 (2), 89-96, 2013

    一般社団法人　日本臓器保存生物医学会

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