Green Tea Extract-induced Acute Hepatotoxicity in Rats
-
- Emoto Yuko
- Pathology II, Kansai Medical University
-
- Yoshizawa Katsuhiko
- Pathology II, Kansai Medical University
-
- Kinoshita Yuichi
- Pathology II, Kansai Medical University
-
- Yuki Michiko
- Pathology II, Kansai Medical University
-
- Yuri Takashi
- Pathology II, Kansai Medical University
-
- Yoshikawa Yutaka
- Department of Health, Sports, and Nutrition, Faculty of Health and Welfare, Kobe Women’s University
-
- Sayama Kazutoshi
- Faculty of Agriculture, Shizuoka University
-
- Tsubura Airo
- Pathology II, Kansai Medical University
この論文をさがす
抄録
Although green tea is considered to be a healthy beverage, hepatotoxicity associated with the consumption of green tea extract has been reported. In the present study, we characterized the hepatotoxicity of green tea extract in rats and explored the responsible mechanism. Six-week-old IGS rats received a single intraperitoneal (ip) injection of 200 mg/kg green tea extract (THEA-FLAN 90S). At 8, 24, 48 and 72 hrs and 1 and 3 months after exposure, liver damage was assessed by using blood-chemistry, histopathology, and immunohistochemistry to detect cell death (TUNEL and caspase-3) and proliferative activity (PCNA). Analyses of malondialdehyde (MDA) in serum and the liver and of MDA and thymidine glycol (TG) by immunohistochemistry, as oxidative stress markers, were performed. Placental glutathione S-transferase (GST-P), which is a marker of hepatocarcinogenesis, was also immunohistochemically stained. To examine toxicity at older ages, 200 mg/kg green tea extract was administered to 18-wk-old female rats. In 6-wk-old rats, 12% of males and 50% of females died within 72 hrs. In 18-wk-old rats, 88% died within 72 hrs. The serum levels of aspartate aminotransferase, alanine aminotransferase and/or total bilirubin increased in both males and females. Single-cell necrosis with positive signs of TUNEL and caspase-3 was seen in perilobular hepatocytes from 8 hrs onward in all lobular areas. PCNA-positive hepatocytes increased at 48 hrs. MDA levels in the serum and liver tended to increase, and MDA- and TG-positive hepatocytes were seen immunohistochemically. GST-P–positive hepatocellular altered foci were detected in one female rat at the 3-month time point. In conclusion, a single injection of green tea extract induced acute and severe hepatotoxicity, which might be associated with lipid peroxidation and DNA oxidative stress in hepatocytes.
収録刊行物
-
- Journal of Toxicologic Pathology
-
Journal of Toxicologic Pathology 27 (3+4), 163-174, 2014
日本毒性病理学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001204416177152
-
- NII論文ID
- 130004713011
-
- NII書誌ID
- AN10232280
-
- ISSN
- 1881915X
- 09149198
-
- NDL書誌ID
- 025899745
-
- PubMed
- 25378801
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可