Introduction of a Single Transporter Gene ABCA3 Directs RLE-6TN to More Type Ⅱ-like Alveolar Epithelial Cells

• Takano Mikihisa

  Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical & Health Sciences, Hiroshima University

• Yamamoto Chieko

  Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical & Health Sciences, Hiroshima University

• Sambuichi Keisuke

  Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical & Health Sciences, Hiroshima University

• Oda Keisuke

  Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical & Health Sciences, Hiroshima University

• Nagai Junya

  Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical & Health Sciences, Hiroshima University

• Shimamoto Akira

  Department of Cellular and Molecular Biology, Graduate School of Biomedical & Health Sciences, Hiroshima University

• Tahara Hidetoshi

  Department of Cellular and Molecular Biology, Graduate School of Biomedical & Health Sciences, Hiroshima University

• Yumoto Ryoko

  Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical & Health Sciences, Hiroshima University

書誌事項

タイトル別名

• 【Original Contribution】 Introduction of a Single Transporter Gene <i>ABCA3</i> Directs RLE-6TN to More Type II-like Alveolar Epithelial Cells
• Introduction of a single transport gene ABCA3 directs RLE-6TN to more type II-like alveolar epithelial cells

抄録

RLE-6TN is a cell line having similar characteristics with those in alveolar type II epithelial cells. However, the development of lamellar bodies, characteristic intracellular structures in type II cells, in RLE-6TN cells is not enough. In this study, RLE-6TN cells were transfected with rat ABCA3 gene using a retroviral vector, and phenotypical changes were examined. The expression of ABCA3 mRNA and protein as well as the number and size of lamellar body-like structures was increased in RLE/ABCA3 compared with RLE/Vector cells. Surprisingly, not only the expression of ABCA3 mRNA but also the expression of other mRNAs such as SP-A and PEPT2, that are highly expressed in type II cells than in type I cells, was increased in RLE/ABCA3 cells. In contrast, the expression of mRNAs that are highly expressed in type I cells was hardly increased by ABCA3 transfection. In addition, albumin uptake activity, that is much higher in type II cells than in type I cells, was also enhanced in RLE/ABCA3 cells. These results suggest that the introduction of a single transporter gene ABCA3 would direct RLE-6TN to more type II-like cells, and RLE/ABCA3 cells may be useful as a novel in-vitro model of alveolar type II epithelial cells.

収録刊行物

• 膜

  膜 38 (5), 246-253, 2013

  日本膜学会

キーワード

• alveolar epithelial cells
• type Ⅱ cells
• ABCA3
• lamellar body
• albumin uptake

詳細情報

• CRID: 1390001206422871552
• NII論文ID: 130004780906
• NII書誌ID: AN0023215X
• DOI: 10.5360/membrane.38.246
• COI: 1:CAS:528:DC%2BC3sXhvFSks7nL
• ISSN: 18846440
• ISSN: 03851036
• NDL書誌ID: 024914495
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024914495
• Web Site: https://www.jstage.jst.go.jp/article/membrane/38/5/38_246/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2014116390
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可