Failure to Protect Yellowtail Seriola quinqueradiata against Nocardiosis by Vaccination with a Recombinant Protein and pcDNA4 Expression Vector of Edwardsiella tarda Glyceraldehyde-3-phosphate Dehydrogenase

• Imajoh Masayuki

  Laboratory of Fish Disease, Faculty of Agriculture, Kochi University

• Tamura Kazuki

  Laboratory of Fish Disease, Faculty of Agriculture, Kochi University

• Oguro Kazuki

  Laboratory of Fish Disease, Faculty of Agriculture, Kochi University

• Kurihara Sumire

  Laboratory of Fish Disease, Faculty of Agriculture, Kochi University

• Yamane Jin

  Laboratory of Fish Disease, Faculty of Agriculture, Kochi University

• Shimizu Masato

  Laboratory of Fish Disease, Faculty of Agriculture, Kochi University

• Oshima Syun-ichirou

  Graduate School of Kuroshio Science, Kochi University

• Kawai Kenji

  The United Graduate School of Agricultural Sciences, Ehime University

書誌事項

タイトル別名

• Failure to Protect Yellowtail <i>Seriola quinqueradiata</i> against Nocardiosis by Vaccination with a Recombinant Protein and pcDNA4 Expression Vector of <i>Edwardsiella tarda</i> Glyceraldehyde-3-phosphate Dehydrogenase

抄録

Vaccination with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Edwardsiella tarda has been demonstrated to have cross-protection against some other bacterial pathogens. We prepared a recombinant protein and pcDNA4 expression vector of GAPDH of E. tarda and injected those to 0-age yellowtail Seriola quinqueradiata to evaluate their protective efficacies against Nocardia seriolae. The 60-d survival rates ranged from 0% to 6.7% among vaccinated and control groups, suggesting that the protein and expression vector of E. tarda GAPDH had no protective effect against nocardiosis. However, the copy numbers of the 16S ribosomal RNA gene of N. seriolae were significantly lower in the gill, kidney, and spleen of vaccinated fish 9 and 18 days post-infection compared with those in the control group that was also challenged with N. seriolae. Additionally, the CC chemokine, major histocompatibility complex class II α antigen, interleukin 1β, and immunoglobulin M genes were significantly upregulated in the vaccinated groups. Four predicted B-cell epitopes of GAPDH in E. tarda, which may play an important role in cross-protection, differed in amino acid sequences from those of GAPDH in N. seriolae. This may explain the lack of a protective effect of E. tarda GAPDH against N. seriolae.

収録刊行物

• 魚病研究

  魚病研究 49 (4), 151-158, 2014

  日本魚病学会

キーワード

• <i>Edwardsiella tarda</i>
• <i>Nocardia seriolae</i>
• GAPDH
• vaccine
• adjuvant
• yellowtail
• B-cell epitope

詳細情報

• CRID: 1390001206461983488
• NII論文ID: 130004785813
• NII書誌ID: AN00063165
• DOI: 10.3147/jsfp.49.151
• ISSN: 18817335
• ISSN: 0388788X
• NDL書誌ID: 026000839
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026000839
• 本文言語コード: ja
• データソース種別:
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