Molecular cloning and characterization of a Pichia pastoris ortholog of the yeast Golgi GDP-mannose transporter gene
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- Arakawa Kumiko
- Department of Biotechnology, University of Tokyo
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- Abe Masato
- Department of Biotechnology, University of Tokyo
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- Noda Yoichi
- Department of Biotechnology, University of Tokyo
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- Adachi Hiroyuki
- Department of Biotechnology, University of Tokyo
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- Yoda Koji
- Department of Biotechnology, University of Tokyo
抄録
There are two structural profiles in the yeast Golgi. The Golgi of Saccharomyces cerevisiae is composed of a number of vesicular compartments dispersed in the cytoplasm as recognized by a large number of Golgi marker proteins. In contrast, the Golgi of Pichia pastoris was reported to be organized in a small number of stacked cisternae located near the transitional endoplasmic reticulum (tER) sites by electron microscopy and immunofluorescent staining of a few marker proteins. The guanosine diphosphate (GDP)-mannose transporter (GMT) is an essential component in the yeast Golgi apparatus. We isolated an ortholog of the GMT gene of P. pastoris and visualized the gene product by epitope tagging to verify the structural characteristics of the Golgi. The tagged product in P. pastoris cell was observed in rod-like compartments in which Och1 mannosyltransferase was also found and the tER marker Sec12 and Sec13 proteins localized very close to them. The present results add further evidence of the restricted localization of the Golgi in P. pastoris cell.
収録刊行物
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- The Journal of General and Applied Microbiology
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The Journal of General and Applied Microbiology 52 (3), 137-145, 2006
公益財団法人 応用微生物学・分子細胞生物学研究奨励会
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詳細情報 詳細情報について
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- CRID
- 1390282679124232704
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- NII論文ID
- 130004802064
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- COI
- 1:CAS:528:DC%2BD28Xpt1arsL0%3D
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- ISSN
- 13498037
- 00221260
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可