Quantitative Comparison of Caffeoylquinic Acids and Flavonoids in Chrysanthemum morifolium Flowers and Their Sulfur-Fumigated Products by Three-Channel Liquid Chromatography with Electrochemical Detection

• Chen Liangmian

  School of Pharmacy, Tokyo University of Pharmacy and Life Sciences Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine

• Kotani Akira

  School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

• Kusu Fumiyo

  School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

• Wang Zhimin

  Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine

• Zhu Jingjing

  Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine

• Hakamata Hideki

  School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

書誌事項

タイトル別名

• Quantitative Comparison of Caffeoylquinic Acids and Flavonoids in <i>Chrysanthemum morifolium</i> Flowers and Their Sulfur-Fumigated Products by Three-Channel Liquid Chromatography with Electrochemical Detection

抄録

For the determination of seven caffeoylquinic acids [neochlorogenic acid (NcA), cryptochlorogenic acid (CcA), chlorogenic acid (CA), caffeic acid (CfA), isochlorogenic acid A (Ic A), isochlorogenic acid B (Ic B), isochlorogenic acid C (Ic C)] and two flavonoids [luteolin 7-O-glucoside (LtG) and luteolin (Lt)], a three-channel liquid chromatography with electrochemical detection (LC-3ECD) method was established. Chromatographic peak heights were proportional to each concentration, ranging from 2.5 to 100 ng/mL for NcA, CA, CcA, and CfA, and ranging from 2.5 to 250 ng/mL for LtG, Ic B, Ic A, Ic C, and Lt, respectively. The present LC-3ECD method was applied to the quantitative analysis of caffeoylquinic acids and flavonoids in four cultivars of Chrysanthemum morifolium flowers and their sulfur-fumigated products. It was found that 60% of LtG and more than 47% of caffeoylquinic acids were lost during the sulfur fumigation processing. Sulfur fumigation showed a destructive effect on the C. morifolium flowers. In addition, principle component analyses (PCA) were performed using the results of the quantitative analysis of caffeoylquinic acids and flavonoids to compare the “sameness” and “differences” of these analytes in C. morifolium flowers and the sulfur-fumigated products. PCA score plots showed that the four cultivars of C. morifolium flowers were clearly classified into four groups, and that significant differences were also found between the non-fumigated C. morifolium flowers and the sulfur-fumigated products. Therefore, it was demonstrated that the present LC-3ECD method coupled with PCA is applicable to the variation analysis of different C. morifolium flower samples.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 63 (1), 25-32, 2015

  公益社団法人 日本薬学会

キーワード

• <i>Chrysanthemum morifolium</i> flower
• caffeoylquinic acid
• flavonoid
• sulfur-fumigation
• electrochemical detection

詳細情報

• CRID: 1390001204176468480
• NII論文ID: 130004803751
• NII書誌ID: AA00602100
• DOI: 10.1248/cpb.c14-00515
• ISSN: 13475223
• ISSN: 00092363
• NDL書誌ID: 026000316
• PubMed: 25743191
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026000316
• Web Site: https://www.jstage.jst.go.jp/article/cpb/63/1/63_c14-00515/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015278772
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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