Postnatal development of inhibitory synaptic transmission to superior salivatory neurons in rats

• Mitoh Yoshihiro

  Department of Oral Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

• Funahashi Makoto

  Department of Oral Physiology, Hokkaido University Graduate School of Dental Medicine

• Fujita Masako

  Department of Oral Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

• Kobashi Motoi

  Department of Oral Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

• Matsuo Ryuji

  Department of Oral Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

抄録

The primary parasympathetic center of the submandibular and sublingual salivary glands is the superior salivatory (SS) nucleus, and its neurons receive excitatory (glutamatergic) and inhibitory (GABAergic and glycinergic) synaptic transmissions in rats. In the present study, we focused on the postnatal development of inhibitory transmission to SS neurons. Gramicidin-perforated whole-cell patch-clamp recordings were performed in rat brainstem slices on postnatal day 2 (P2)-P14. Developmental changes in the intracellular Cl^- concentration ([Cl^-]_in) were examined based on the reversal potentials of total inhibitory postsynaptic currents (GABAergic plus glycinergic), which were evoked by electrical stimulation near the recording neuron. The [Cl^-]_in in the P8-P14 group was significantly lower than in the P2-P7 group. The effect of GABA application at the resting potentials changed from depolarization to hyperpolarization around P8, suggesting that SS neurons acquired mature inhibitory systems around P8. The period at which GABA responses change from excitatory to inhibitory in SS neurons was discussed compared with those of the forebrain, brainstem, and spinal neurons. J. Med. Invest. 56 Suppl.: 270-272, December, 2009

収録刊行物

• The Journal of Medical Investigation

  The Journal of Medical Investigation 56 (Supplement), 270-272, 2009

  国立大学法人　徳島大学医学部

キーワード

• superior salivatory neurons
• development
• GABAergic
• glycinergic
• gramicidin-perforated patch clamp

詳細情報

• CRID: 1390001204242865408
• NII論文ID: 130004822613
• NII書誌ID: AA11166929
• DOI: 10.2152/jmi.56.270
• ISSN: 13496867
• ISSN: 13431420
• Web Site: https://repo.lib.tokushima-u.ac.jp/111347
• Web Site: http://www.jstage.jst.go.jp/article/jmi/56/Supplement/56_Supplement_270/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010325493
• 本文言語コード: en
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