Spontaneous Ca2+ oscillations via purinergic receptors elicit transient cell swelling in rat parotid ducts

• Shitara Akiko

  Department of Pharmacology, School of Dentistry, Health Sciences University of Hokkaido Department of Biochemistry, School of Dentistry, Health Sciences University of Hokkaido

• Tanimura Akihiko

  Department of Pharmacology, School of Dentistry, Health Sciences University of Hokkaido

• Sato Atsuko

  First Department of Oral Anatomy, Fukuoka Dental College

• Tojyo Yosuke

  Department of Pharmacology, School of Dentistry, Health Sciences University of Hokkaido

抄録

Rat parotid ductal cells were found to exhibit spontaneous Ca²⁺ oscillations. These oscillatory Ca²⁺ responses were observed during continuous perfusion with physiological salt solution at 37°C in the absence of calcium mobilizing agonist stimulation. These Ca²⁺ oscillations were completely blocked by the purinergic receptor inhibitors, pyridoxal phosphate-6-azo (benzene-2,4-disulfonic acid) (PPADS) and suramin, but were not blocked by the muscarinic antagonist, atropine, nor the α-adrenergic antagonist, phentolamine. Simultaneous observation with fura-2 fluorescence and differential interference contrast (DIC) images showed that the spontaneous elevations of [Ca²⁺]i were well correlated with the shape changes of the ductal cells. Using a plasma membrane fluorescence probe, we found that the changes in DIC images reflected spontaneous cell swelling of ductal cells. Electron microscopic analysis after Ca²⁺ imaging indicated that the spontaneously oscillating duct cells contained numerous granules at the luminal side, which is characteristic of the granular duct cells. These results indicate that the spontaneous [Ca²⁺]i increase occurs through purinergic receptors, and activates Ca²⁺-dependent ion transporters and/or channels. Our findings present the possibility that spontaneous Ca²⁺ oscillations via purinergic receptors are involved in the regulation of the electrolyte composition of saliva in resting states. J. Med. Invest. 56 Suppl.: 377-380, December, 2009

収録刊行物

• The Journal of Medical Investigation

  The Journal of Medical Investigation 56 (Supplement), 377-380, 2009

  国立大学法人　徳島大学医学部

キーワード

• salivary duct
• spontaneous Ca<SUP>2+</SUP>
• oscillation
• purinergic receptors
• cell swelling

詳細情報

• CRID: 1390001204243068928
• NII論文ID: 130004822645
• NII論文ID: 120006485169
• NII書誌ID: AA11166929
• DOI: 10.2152/jmi.56.377
• ISSN: 13496867
• ISSN: 13431420
• Web Site: http://repo.lib.tokushima-u.ac.jp/111481
• Web Site: http://www.jstage.jst.go.jp/article/jmi/56/Supplement/56_Supplement_377/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010325525
• 本文言語コード: en
• データソース種別:
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  • IRDB
  • Crossref
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