Analysis of the monosaccharide components in Angelica polysaccharide by high performance liquid chromatography

• YANG Xingbin

  Department of Pharmacology, the Fourth Military Medical University

• ZHAO Yan

  Department of Chemistry, the Fourth Military Medical University

• WANG Qingwei

  Department of Pharmacology, the Fourth Military Medical University

• WANG Haifang

  Department of Pharmacology, the Fourth Military Medical University

• MEI Qibing

  Department of Pharmacology, the Fourth Military Medical University

書誌事項

タイトル別名

• Analysis of the Monosaccharide Components in Angelica Polysaccharides by High Performance Liquid Chromatography

抄録

An analytical method of on-line high performance liquid chromatography (HLPC) was developed to simultaneously separate and identify the monosaccharide composition of three Angelica polysaccharide fractions (APF), named APF1, APF2 and APF3. In this method, APF were hydrolyzed into component monosaccharides and subsequently labeled with 1-phenyl-3-methyl-5-pyrazolone (PMP), and then the labeled monosaccharide derivatives were separated by a reverse-phase C₁₈ column and monitored by UV absorbance at 250 nm. The results showed that nine monosaccharide derivatives have been well separated by HPLC under optimized conditions and the composition analysis of monosaccharides from APF samples could be achieved using acid hydrolysis and a set of monosaccharide standards. With this method, the within-day and day to day precisions of the composition determinations were 3.41 - 4.87% and 3.12 - 4.93% (RSD), respectively. The method was successfully applied to the determination of the component monosaccharides of Angelica polysaccharides.

収録刊行物

• Analytical Sciences

  Analytical Sciences 21 (10), 1177-1180, 2005

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390001204257194880
• NII論文ID: 130004826346
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.21.1177
• COI: 1:CAS:528:DC%2BD2MXhtFGmtbrK
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 7467375
• PubMed: 16270574
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7467375
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.21.1177.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.21.1177/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/21/10/21_10_1177/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可