Electrochemical Detection of Sugar-related Compounds Using Boron-doped Diamond Electrodes
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- HAYASHI Tomohisa
- School of Pharmacy, Nihon University
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- SAKURADA Ikuo
- Comet Co.
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- HONDA Kensuke
- Department of Biological Science and Chemistry, Faculty of Science, Yamaguchi University
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- MOTOHASHI Shigeyasu
- School of Pharmacy, Nihon University
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- UCHIKURA Kazuo
- School of Pharmacy, Nihon University
抄録
Electrochemical detection of sugar-related compounds was conducted using a boron-doped diamond (BDD) electrode as a detector for flow-injection analysis (FIA). Sugar-related compounds oxidize at high applied potentials, for which the BDD electrode is suitable for electrochemical measurements. Conditions for an FIA system with a BDD detector were optimized, and the following detection limits were achieved for sugar-related compounds: monosaccharides, 25 – 100 pmol; sugar alcohols, 10 pmol; and oligosaccharides, 10 pmol. The detection limit for monosaccharide D-glucose (Glu) was 105 pmol (S/N = 3). A linear range was acquired from the detection limit to 50 nmol, and the relative standard deviation was 0.65% (20 nmol, n = 6). A high-performance liquid chromatography (HPLC) column was added to the system between the sample injector and the detector and detection limits to the picomole level were achieved, which is the same for the HPLC system and the FIA system. The electrochemical oxidation reaction of Glu was examined using cyclic voltammetry with the BDD detector. The reaction proved to be irreversible, and proceeded according to the following two-step mechanism: (1) application of a high potential (2.00 V vs. Ag/AgCl) to the electrode causes water to electrolyze on the electrode surface with the simultaneous generation of a hydroxyl radical on the surface, and (2) the hydroxyl radical indirectly oxidizes Glu. Thus, Glu can be detected by an increase in the oxidation current caused by reactions with hydroxy radicals.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 28 (2), 127-127, 2012
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679238432768
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- NII論文ID
- 130004827193
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- ISSN
- 13482246
- 09106340
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可