Simultaneous Quantitation of Alpha-ketoglutaric Acid and 5-Hydroxymethylfurfural in Plasma by HPLC with UV and Fluorescence Detection

• DONNARUMMA Fabrizio

  Institute of Pharmaceutical Sciences, University of Graz

• WINTERSTEIGER Reinhold

  Institute of Pharmaceutical Sciences, University of Graz

• SCHOBER Margot

  Institute of Pharmaceutical Sciences, University of Graz

• GREILBERGER Joachim

  Institute of Physiological Chemistry, Medical University of Graz

• MATZI Veronika

  Department of Thoracic and Hyperbaric Surgery, Medical University of Graz

• MAIER Alfred

  Department of Thoracic and Hyperbaric Surgery, Medical University of Graz

• SCHWARZ Michaela

  Department of Transplant Surgery, LKH-University Clinic Graz

• ORTNER Astrid

  Institute of Pharmaceutical Sciences, University of Graz

抄録

Alpha-ketoglutaric acid (KG) and hydroxymethylfurfural (HMF) are currently being investigated in clinical trials as an approach in targeted cancer therapy. Hence, a method for the simultaneous determination of KG and HMF in plasma has been developed. Due to the strongly discriminative chemical properties of KG and HMF, SPE purification is performed using an ion-exchange cartridge to separate KG, and a hydrophobic polymeric cartridge to separate HMF. The cartridges are connected together for several steps, thus resulting in a quicker approach for the purification of plasma samples. The derivatization step is based on the reaction of the carbonyl groups of KG and HMF with dansylhydrazine (DNSH) catalyzed by trifluoroacetic acid. The formed derivatives could be separated by reversed-phase LC on a C8-column, and analyzed by UV and fluorescence detection in a single run using a gradient program. The obtained results show good reproducibility, specificity, and detection limits down to the low picomole range.

収録刊行物

• Analytical Sciences

  Analytical Sciences 29 (12), 1177-1182, 2013

  社団法人 日本分析化学会

キーワード

• Ketoglutaric acid
• hydroxymethylfurfural
• HPLC
• UV and fluorescence detection
• SPE

詳細情報

• CRID: 1390282679238604544
• NII論文ID: 130004827324
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.29.1177
• COI: 1:STN:280:DC%2BC2c3ls1OrtQ%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 025074587
• PubMed: 24334984
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025074587
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.29.1177.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.29.1177/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/29/12/29_1177/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2014316308
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可