Sequence-selective Modification of DNA Cytosine by Using Junction-forming DNA Probes and Its Application to the Detection of Single Cytosine Methylation

• TAKANASHI Kenta

  Graduate School of Bionics, Computer and Media Sciences, Tokyo University of Technology

• KATO Teru

  Graduate School of Bionics, Computer and Media Sciences, Tokyo University of Technology

抄録

Methylated DNA plays an important role in epigenetic gene regulation, and could therefore serve as a potentially promising biomarker for the diagnosis of cancer and other diseases. Therefore, the development of a simple method for analyzing cytosine methylation in a target gene is required. Here, we report on a new method for the sequence-selective chemical modification of a single cytosine in single-stranded DNA (ssDNA). This method is based on the formation of a DNA three-way junction of the ssDNA and two ssDNA probes, and was successfully applied for a simple polymerase chain reaction (PCR)-based assay for ‘pinpoint’ methylation analysis.

収録刊行物

• Analytical Sciences

  Analytical Sciences 30 (3), 371-376, 2014

  社団法人 日本分析化学会

キーワード

• DNA methylation
• epigenetics
• three-way junction (TWJ)
• bisulfite
• aminooxy reagent
• <i>Taq</i> DNA polymerase
• primer extension
• PCR

詳細情報

• CRID: 1390001204260632320
• NII論文ID: 130004827420
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.30.371
• COI: 1:STN:280:DC%2BC2crhslOhtA%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 025345515
• PubMed: 24614732
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025345515
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.30.371.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.30.371/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/30/3/30_371/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2014236665
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可