Ultrasensitive Biosensor for Detection of Organophosphorus Pesticides Based on a Macrocycle Complex/Carbon Nanotubes Composite and 1-Methyl-3-octylimidazolium Tetrafluoroborate as Binder Compound

• PEREIRA Neuma das Mercês

  Departamento de Química, UFVJM

• OLIVEIRA Fernando Mota de

  Departamento de Química, UFVJM

• PEREIRA Nara Rúbia

  Instituto de Ciências Exatas e Biológicas, UFOP

• VERLY Rodrigo Moreira

  Departamento de Química, UFVJM

• SOUTO Dênio Emanuel Pires

  Instituto de Química, UNICAMP

• KUBOTA Lauro Tatsuo

  Instituto de Química, UNICAMP

• TANAKA Auro Atsushi

  Departamento de Química, UFMA

• DAMOS Flavio Santos

  Departamento de Química, UFMA

• LUZ Rita de Cássia Silva

  Departamento de Química, UFMA

抄録

This work describes the highly sensitive detection of organophosphorus pesticides employing the cobalt(II) 4,4,4,4-tetrasulfo-phthalocyanine (CoTSPc) macrocycle complex, carbon nanotubes (CNT), and 1-methyl-3-octylimidazolium tetrafluoroborate (OMIM[BF₄]). The technique is based on enzyme acetylcholinesterase (AChE) inhibition. The composite was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, and amperometry. The AChE was immobilized on the composite electrode surface by cross-linking with glutaraldehyde and chitosan. The synergistic action of the CoTSPc/CNT/OMIM[BF₄] composite showed excellent electrocatalytic activity, with a low applied potential for the amperometric detection of thiocholine (TCh) at 0.0 V vs. Ag/AgCl. The calculated catalytic rate constant, k_cat, was 3.67 × 10³ mol⁻¹ L s⁻¹. Under the optimum conditions, the inhibition rates of these pesticides were proportional to their concentrations in the ranges of 1.0 pmol L⁻¹ to 1.0 nmol L⁻¹ (fenitrothion), 2.0 pmol L⁻¹ to 8.0 nmol L⁻¹ (dichlorvos), and 16 pmol L⁻¹ to 5.0 nmol L⁻¹ (malathion).

収録刊行物

• Analytical Sciences

  Analytical Sciences 31 (1), 29-35, 2015

  社団法人 日本分析化学会

キーワード

• Biosensor
• cetylcholinesterase
• CoTsPc
• CNT
• OMIM[BF₄]
• OP pesticides

詳細情報

• CRID: 1390001204259772032
• NII論文ID: 130004827443
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.31.29
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 026048544
• PubMed: 25792271
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026048544
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.31.29.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.31.29/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/31/1/31_29/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可