Self-assembled Nanowire Arrays as Three-dimensional Nanopores for Filtration of DNA Molecules

• RAHONG Sakon

  Institute of Innovation for Future Society, Nagoya University FIRST Research Center for Innovative Nanobiodevices, Nagoya University

• YASUI Takao

  FIRST Research Center for Innovative Nanobiodevices, Nagoya University Department of Applied Chemistry, Graduate School of Engineering, Nagoya University

• YANAGIDA Takeshi

  The Institute of Scientific and Industrial Research, Osaka University

• NAGASHIMA Kazuki

  The Institute of Scientific and Industrial Research, Osaka University

• KANAI Masaki

  The Institute of Scientific and Industrial Research, Osaka University

• MENG Gang

  The Institute of Scientific and Industrial Research, Osaka University

• HE Yong

  The Institute of Scientific and Industrial Research, Osaka University

• ZHUGE Fuwei

  The Institute of Scientific and Industrial Research, Osaka University

• KAJI Noritada

  FIRST Research Center for Innovative Nanobiodevices, Nagoya University Department of Applied Chemistry, Graduate School of Engineering, Nagoya University

• KAWAI Tomoji

  The Institute of Scientific and Industrial Research, Osaka University

• BABA Yoshinobu

  FIRST Research Center for Innovative Nanobiodevices, Nagoya University Department of Applied Chemistry, Graduate School of Engineering, Nagoya University Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

抄録

Molecular filtration and purification play important roles for biomolecule analysis. However, it is still necessary to improve efficiency and reduce the filtration time. Here, we show self-assembled nanowire arrays as three-dimensional (3D) nanopores embedded in a microfluidic channel for ultrafast DNA filtration. The 3D nanopore structure was formed by a vapor-liquid-solid (VLS) nanowire growth technique, which allowed us to control pore size of the filtration material by varying the number of growth cycles. λ DNA molecules (48.5 kbp) were filtrated from a mixture of T4 DNA (166 kbp) at the entrance of the 3D nanopore structure within 1 s under an applied electric field. Moreover, we observed single DNA molecule migration of T4 and λ DNA molecules to clarify the filtration mechanism. The 3D nanopore structure has simplicity of fabrication, flexibility of pore size control and reusability for biomolecule filtration. Consequently it is an excellent material for biomolecular filtration.

収録刊行物

• Analytical Sciences

  Analytical Sciences 31 (3), 153-157, 2015

  社団法人 日本分析化学会

キーワード

• Nanowire
• 3D nanopore structure
• biomolecular filtration

詳細情報

• CRID: 1390282679236975104
• NII論文ID: 130004827469
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.31.153
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 026247253
• PubMed: 25765268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026247253
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.31.153.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.31.153/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/31/3/31_153/_pdf
• 本文言語コード: en
• データソース種別:
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