Decrease of Both Cofilin and LIM Kinase Phosphorylation in the Skeletal Muscles of Immobilization-induced Atrophy Rats
-
- Kim Mee-Young
- Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea
-
- Kim Ju-Hyun
- Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea
-
- Lee Jeong-Uk
- Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea
-
- Lee Lim-Kyu
- Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea
-
- Yang Seung-Min
- Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea
-
- Jeon Hye-Joo
- Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea
-
- Lee Won-Deok
- Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea
-
- Noh Ji-Woong
- Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea
-
- Lee Tae-Hyun
- Department of Combative Martial Arts Training, College of Martial Arts, Yongin University, Republic of Korea
-
- Kwak Taek-Yong
- Taekwondo Instructor Education, College of Martial Arts, Yongin University, Republic of Korea
-
- Kim Bokyung
- Institute of Functional Genomics, Department of Physiology, School of Medicine, Konkuk University, Republic of Korea
-
- Kim Junghwan
- Department of Physical Therapy, College of Public Health and Welfare, Yongin University: Yongin 449-714, Republic of Korea
この論文をさがす
抄録
[Purpose] Immobilization-induced atrophy is a general phenomenon caused by prolonged muscle disuse associated with orthopaedic conditions. However, changes in the phosphorylation of atrophy-related cofilin and LIM kinases are still poorly understood. In this study, we examined whether or not phosphorylation of cofilin and LIM kinases is altered in the skeletal muscles of rats after 3, 7, 14, and 21 days of cast immobilization. [Methods] We used two-dimensional gel electrophoresis, mass spectrometry, and western blotting to examine protein expression and phosphorylation in atrophied rat gastrocnemius muscles. [Results] The expression of the cofilin was detected in gastrocnemius muscle strips using proteomic analysis. Cast immobilization after 3, 7, 14, and 21 days significantly diminished the phosphorylation of cofilin and LIM kinases. [Conclusion] The present results suggest that cast immobilization-induced atrophy may be in part related to changes in the phosphorylation of cofilin and LIM kinases in rat skeletal muscles.
収録刊行物
-
- Ｊ Ｐｈｙｓ Ｔｈｅｒ Ｓｃｉ
-
Ｊ Ｐｈｙｓ Ｔｈｅｒ Ｓｃｉ 26 (3), 355-357, 2014
理学療法科学学会