細胞挙動に基づく細胞寿命の定量化および増殖ポテンシャル評価

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  • Evaluation of Cell Growth Potential and Quantitative of Cellular Life-span Based on Cell Behavior in Cell Culture Process

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An evaluation system for the management of cell culture process is required to noninvasively evaluate the cellular growth potential in the cell culture process. In cell culture process, cell behavior is influenced by the state of the cell potential. Therefore, it is necessary to evaluate accordance with the change of cell behavior. In this study, evaluation method was developed with measuring the cell behavior of observed individual cells at elapse time. The passage culture of HPASMC cells was shown that the ratio of individual cells with fast migration (over 72 μm/hour) decreased with increased of culture passage. Cells in an early culture passage indicated the average projected area increased over time. Moreover, the cell migration flux was newly calculated as evaluation index for quantification of cell growth potential. The migration flux of individual cells decreased with decreased residual number of cell divisions. In conclusions, it was suggested that migration flux can be evaluated cellular potential to measure migration rate and projected area at elapse time in cell culture process. Additionally, it was suggested that cell migration flux was useful for management of cell culture process and quality evaluation.

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