αB-Crystallin-like Immunoreactivity Localized at Both the M-and Z-Lines in Isolated Syrian Hamster Ventricular Myocytes

• Ono Kageyoshi

  Division of Xenobiotic Metabolism and Disposition, National Institute of Health Sciences

• Imazawa Takayoshi

  Division of Pathology, National Institute of Health Sciences

• Sakamoto Aiji

  Division of Biotechnology, National Cardiovascular Center Research Institute Form and Function, PRESTO, Japan Science and Technology Corporation

抄録

αB-Crystallin (CryAB) is a major stress protein in cardiac myocytes. Under pathological stress such as ischemia, it acts as a molecular chaperone to suppress protein aggregation at the Z-line and at the I-band region of myofibril and at the intercalated disk. We report here that CryAB-like immunoreactivity also exists as a relatively weak but distinct signal at the M-line in isolated Syrian hamster ventricular myocytes, using a new laser confocal immunofluorescence microscope with high resolution. Our electron microscopic examination revealed that mitochondria in the isolated myocytes carried evidence of mild ischemia. Immunoblot analysis using a polyclonal antibody against CryAB detected a single protein with molecular mass of 21kDa in Syrian hamster left ventricle. The novel localization of CryAB-like immunoreactivity in the cardiac myofibril and its possible relation to ischemia may shed light on a yet to be unraveled chaperonic function of this stress protein in the heart.

収録刊行物

• bioimages

  bioimages 10 (2), 75-79, 2002

  日本バイオイメージング学会

キーワード

• αB-Crystallin
• heart
• laser confocal microscope
• M-line
• ischemia
• hamster

詳細情報

• CRID: 1390001204437551104
• NII論文ID: 130004845955
• DOI: 10.11169/bioimages.10.75
• ISSN: 09192719
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可