A Novel β-1,4-mannanase Isolated from Paenibacillus polymyxa KT551

• Hori Kazuyuki

  Akita Research Institute of Food & Brewing

• Kawabata Yasuyuki

  Department of Food Science and Nutrition, Osaka Shoin Women's University

• Nakazawa Yozo

  Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture

• Nishizawa Makoto

  Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture

• Toeda Kazuki

  Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture

書誌事項

タイトル別名

• A Novel β-1,4-mannanase Isolated from <i>Paenibacillus polymyxa</i> KT551

抄録

A β-1,4-mannanase producing bacterium was isolated from soil collected in Akita Prefecture, Japan. The bacterium was identified as Paenibacillus polymyxa KT551 and was shown to produce a novel β-1,4-mannanase. The novelty of the enzyme was established by its N-terminal amino acid sequence, molecular weight and isoelectric point. The isolated β-1,4-mannanase showed activity against mannotetraose, mannopentaose and mannohexaose to produce mannobiose, mannotriose and mannotetraose. However, the enzyme exhibited no activity against mannobiose and mannotriose. Moreover, the crude enzyme preparation of the bacterium had no or minimal β-mannosidase or α-galactosidase activity. Therefore, the enzyme preparation from P. polymyxa KT551 holds potential for the efficient production of mannooligosaccharides from natural resources of galactomannans.

収録刊行物

• Food Science and Technology Research

  Food Science and Technology Research 20 (6), 1261-1265, 2014

  公益社団法人 日本食品科学工学会

キーワード

• <i>Paenibacillus polymyxa</i>
• β-1,4-mannanase
• mannanooligosaccharides
• β-mannosidase
• α-galactosidase

詳細情報

• CRID: 1390001204459458944
• NII論文ID: 130004848143
• DOI: 10.3136/fstr.20.1261
• ISSN: 18813984
• ISSN: 13446606
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可