Low Level Fluoride Stimulates Epithelial-Mesenchymal Interaction in Oral Mucosa

• He Dawei

  Department of Health Science, Division of Oral Health, Kanagawa Dental College

• Ujjal K. Bhawal

  Department of Health Science, Division of Oral Health, Kanagawa Dental College Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo

• Hamada Nobushiro

  Department of Infection Control, Division of Microbiology, Kanagawa Dental College

• Kuboyama Noboru

  Department of Oral Molecular Pharmacology, Nihon University School of Dentistry at Matsudo

• Abiko Yoshimitsu

  Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo

• Arakawa Hirohisa

  Department of Health Science, Division of Oral Health, Kanagawa Dental College

抄録

Oral mucosa consists of the superficial epithelium and the underlying lamina propria, and it functions as a barrier against exogenous substances. In development, interactions of stem/progenitor cells of the epithelium and mesenchyme are crucial to the morphogenesis of oral mucosa. Our previous work in low level fluoride-induced cell motility of epithelial cells has yielded important clues for periodontal physiology. This study focuses on a working concept of low level fluoride to provide a conducive oral environment for pivotal epithelial-mesenchymal interactions. Cultured human primary gingival epithelial cells treated with 50 μM NaF were investigated by DNA microarray. Quantitative real-time PCR and an in vivo experimental rat skin wound model were employed to confirm the findings obtained with the microarray analysis. Microarray data revealed that low level fluoride-treated human gingival epithelial cells elevated various biological processes. The key proliferation markers, FGF2 and FGF7, and mesenchymal marker, Twist1, expression was up-regulated and quantitative real-time PCR confirmed this observation. Our in vivo study revealed that low concentration of NaF increased FGF2, FGF7, and Twist1 protein expression in fluoride-treated skin wound tissues compared with controls. These results provide new information on low level fluoride-induced epithelial-mesenchymal interactions and may thus aid in the understanding of oral mucosa development.

収録刊行物

• Journal of Hard Tissue Biology

  Journal of Hard Tissue Biology 22 (1), 59-66, 2013

  硬組織再生生物学会

キーワード

• Low level NaF
• Human gingival epithelial cell
• DNA microarray
• Epithelial-mesenchymal interaction

詳細情報

• CRID: 1390282679435394560
• NII論文ID: 130004848411
• NII書誌ID: AA11074332
• DOI: 10.2485/jhtb.22.59
• COI: 1:CAS:528:DC%2BC3sXms1Kgt7o%3D
• ISSN: 1880828X
• ISSN: 13417649
• NDL書誌ID: 024429628
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024429628
• Web Site: https://search.jamas.or.jp/link/ui/2014215535
• 本文言語コード: en
• データソース種別:
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  • NDL
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• 抄録ライセンスフラグ: 使用不可