書誌事項
- タイトル別名
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- Evaluation of the Amount of the Manganese Entry in the Neurons for Activity-induced Manganese-enhanced MRI
- カツドウ イソンセイ マンガンゾウエイ MRI ノ タメ ノ シンケイ サイボウ ナイ エ ノ マンガン リュウニュウリョウ ノ ケントウ
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The activity-induced manganese-enhanced MRI (AIM-MRI) is one of the candidate methods for measuring the neuronal activity noninvasively in vivo. This method has the potential to measure the history of the neuronal activity, since the Mn2+ can enter the active neuron through voltage-dependent Ca2+ channels. However, it has not been clear that the relation between the neuronal activity and the accumulation of Mn2+ in the cell. At first, we confirmed that the longitudinal relaxation time of H+ (T1) measured by means of MRI apparatus was related to the [Mn2+] using the pseudo intracellular phantoms with various concentration of Mn2+. The inverse of T1 (1/T1) was proportional to [Mn2+], thus [Mn2+] can be measured by MRI quantitatively. Next, we investigated the relation between the neuronal activity and the accumulation of Mn2+ in the striatal GABAergic neurons using the method of the Mn2+ quenching of Fura-2 LR, a family of fura fluorescent Ca2+ indicators. The amount of the Ca2+ influx was correlated to the neuronal activity evoked by tetanic stimulations with various frequencies. In the same slice preparation, the amounts of the fluorescence quench induced by the tetanic stimulations under the condition of 50 µM MnCl2 administration were recorded. As a result, the amount of the quench was proportional to the amount of the Ca2+ influx. These results suggested that a cell, which has higher activity, accumulated larger amount of Mn2+ in the neuron. Thus, our results supported that AIM-MRI can measure the history of the neuronal activities in the whole brain noninvasively in vivo.
収録刊行物
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- 電気学会論文誌C(電子・情報・システム部門誌)
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電気学会論文誌C(電子・情報・システム部門誌) 135 (3), 280-284, 2015
一般社団法人 電気学会
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詳細情報 詳細情報について
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- CRID
- 1390001204608104448
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- NII論文ID
- 130004870157
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- NII書誌ID
- AN10065950
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- ISSN
- 13488155
- 03854221
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- NDL書誌ID
- 026245343
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
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- 使用不可