CONTINUOUS WAVE VISIBLE RED (633 nm) LASER ENERGY ENHANCES THE DEGRANULATION OF MAST CELLS: A POSSIBLE PHOTOMEDIATED WOUND HEALING MECHANISM EXAMINED IN VIVO

• Mayayo E

  The Medical Institute of Vilafortuny

• Trelles MA

  The Medical Institute of Vilafortuny

• Miro L

  Pathological Anatomy Service. John XXIII Hospital of Tarragona, and the Histology Unit, Reus School of Medicine, University of Barcelona

• Baudin G.

  Central Service of Nuclear Medicine, Medical Biophysics and Radioimmunologv, Regional University Hospital of Nimes

• CaIderhead RG

  The Medical Institute of Vilafortuny LG Biomedical

抄録

The mast cell in an interesting repostory of a number of pro- and anti-inflammatory mediators, and plays an important role in the inflammatory stage of the wound healing process, and in allergic reactions. The present study was designed to elicit the effect of visible light radiation at 632.8 nm (visible red) on mast cells in vivo using light and transmission electron microscopy. The tongue of the Swiss mouse is rich in mast cells (MC_s). Tongues of animals in two experimental groups were irradiated using a HeNe laser (15 mW & 4 mW, 2.4 J/cm²,for both output powers, λ = 632.8 nm), and a third set of unirradiated animals served as the control. The two output powers were used, with the irradiation time adjusted to produce the same energy density. The possible nonselective degranulation of LLLT-irradiated mast cells was examined quantitatively and morphometrically using optic and electron microscopy. By means of radioimmunoassay, the histamine content of pulverized tongue was evaluated, and the histamine levels in both experimental and control tissue were compared. The irradiated tongue tissue showed a significantly higher histamine level compared with the control. In addition, the level of histamine in the interstitial cellular medium and the degree of other histological changes such as vasodilation was significantly higher following a single irradiation with the HeNe laser at the above settings. The observations from this study may serve as a further indication of the bioactivative effect of low reactive level laser therapy on mast cells, which are an important component of the wound healing process and inflammation control.

収録刊行物

• レーザーセラピー

  レーザーセラピー 15 (1), 31-35, 2006

  国際光治療学会

キーワード

• HeNe laser
• LLLT
• phototherapy
• mast cell degranulation
• cytokines
• photobioactivation

詳細情報

• CRID: 1390001204633125760
• NII論文ID: 130004872333
• DOI: 10.5978/islsm.15.31
• ISSN: 18847269
• ISSN: 08985901
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可