Reduction of IL-20 Expression in Rheumatoid Arthritis by Linear Polarized Infrared Light Irradiation
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- Imaoka Asayo
- Department of Biochemistry, Nihon University School of Dentistry at Matsudo
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- Zhang Lin
- Department of Biochemistry, Nihon University School of Dentistry at Matsudo
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- Kuboyama Noboru
- Department of Pharmacology, Nihon University School of Dentistry at Matsudo
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- Abiko Yoshimitsu
- Department of Biochemistry, Nihon University School of Dentistry at Matsudo
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Background: Low-level laser is being evaluated for treating rheumatoid arthritis (RA). Recently, the linear polarized infrared light (Super Lizer, SL) irradiation may also be useful for RA treatment. However, the molecular mechanism underlying the effectiveness of SL on RA is unclear. It has been IL-20 may involved in RA disease progression.<br>Aim: To understand how SL action, we constructed the experimental model in vitro using human rheumatoid fibroblast-like synoviocyte (MH7A) and collagen induced (CIA) RA rat in vivo. We examined the effect of SL irradiation on IL-20 gene expression in MH7A and IL-20 protein production in CIA rat joints.<br>Materials and methods: MH7A was cultured and challenged with IL-1β, then examined IL-20 and IL-20R mRNA level by DNA microarray. IL-20 protein expression was examined by immunohistochemistry using a specific antibody against rat IL-20.<br>Result: Scatter plot analysis demonstrated that an increase in IL-20 gene expression by IL-1s was reduced by SL irradiation, but IL-20R did not show a significant change. The Immunohistochemical analysis demonstrated a strong IL-20 staining in synovial membrane tissue of CIA rat joint, and SL irradiation significantly reduced the staining.<br>Discussion: Since IL-20 has been identified as an important cytokine in the pathogenesis of RA, the reduction of IL-20 expression by SL irradiation may be one of mechanisms in reduction of inflammation in RA joints by SL irradiation suggesting that SL irradiation may be useful for RA therapy.
収録刊行物
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- レーザーセラピー
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レーザーセラピー 23 (2), 109-114, 2014
国際光治療学会
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詳細情報 詳細情報について
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- CRID
- 1390001204632220032
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- NII論文ID
- 130004872616
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- ISSN
- 18847269
- 08985901
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可