Expression and Localization of Purinergic P2Y12 Receptor in Human Nasal Mucosa

• Shirasaki Hideaki

  Department of Otolaryngology, Sapporo Medical University, School of Medicine

• Kanaizumi Etsuko

  Department of Otolaryngology, Sapporo Medical University, School of Medicine

• Seki Nobuhiko

  Department of Otolaryngology, Sapporo Medical University, School of Medicine

• Fujita Manabu

  Minase Research Institute, Ono Pharmaceutical

• Kikuchi Megumi

  Department of Otolaryngology, Sapporo Medical University, School of Medicine

• Himi Tetsuo

  Department of Otolaryngology, Sapporo Medical University, School of Medicine

書誌事項

タイトル別名

• Localization and Up-Regulation of Cysteinyl Leukotriene-2 Receptor in Human Allergic Nasal Mucosa
• Localization and up-regulation of cysteinyl leukotriene-2 receptor in human nasam mucosa
• Localization and up-regulation of cystenyl leukotriene-2 receptor in human allergic nasal mucosa
• Localization and up-regulation of cysteinyl leukotriene-2 receptor in human nasal mucosa

抄録

Background: Cysteinyl leukotrienes (CysLTs) are lipid mediators that have been implicated in the pathogenesis of allergic rhinitis. Pharmacological studies using CysLTs indicate that 2 classes of receptors exist, namely, CysLT₁ and CysLT₂ receptors. The former class of receptors is sensitive to the CysLT₁ antagonists currently used to treat asthma and allergic rhinitis, and its localization has been previously examined by our group using immunohistochemistry and in situ hybridization techniques. We investigated the expression and localization of the CysLT₂ receptor in human nasal mucosa by western blot and immunohistochemical analyses.<br> Methods: Human turbinates were obtained after turbinectomy from 16 patients with nasal obstruction refractory to medical therapy. To identify the cells expressing the CysLT₂ receptor, double immunostaining was performed by using anti-CysLT₂ receptor antibody and anti-CD31 (endothelial cell) antibody or anti-smooth muscle actin antibody.<br> Results: A 39 kDa band was detected on the western blots of human turbinates samples by using the anti-CysLT₂ receptor antibody. The expression level of the CysLT₂ receptor in patients with nasal allergy was higher than that in patients with non-allergic rhinitis. The immunohistochemical study also showed an intense immunoreactivity for CysLT₂ receptor in both vascular endothelial cells and vascular smooth muscles.<br> Conclusions: The results indicated that the CysLT₂ receptor plays a primary role in the vascular responses in the upper respiratory tract.<br>

収録刊行物

• Allergology International

  Allergology International 62 (2), 223-228, 2013

  一般社団法人日本アレルギー学会

キーワード

• allergic rhinitis
• CysLT₂ receptor
• immunohistochemistry
• Western blot

詳細情報

• CRID: 1390001204634242560
• NII論文ID: 130004873071
• NII論文ID: 130004873068
• DOI: 10.2332/allergolint.12-oa-0490
• DOI: 10.2332/allergolint.12-oa-0496
• COI: 1:STN:280:DC%2BC3svptVaitA%3D%3D
• COI: 1:STN:280:DC%2BC3srnslGjsg%3D%3D
• ISSN: 14401592
• ISSN: 13238930
• PubMed: 23524649
• PubMed: 23612493
• Web Site: http://api.elsevier.com/content/article/PII:S1323893015301325?httpAccept=text/xml
• Web Site: http://api.elsevier.com/content/article/PII:S1323893015301325?httpAccept=text/plain
• Web Site: http://api.elsevier.com/content/article/PII:S1323893015301301?httpAccept=text/xml
• Web Site: http://api.elsevier.com/content/article/PII:S1323893015301301?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/2014181067
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • PubMed
  • CiNii Articles
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