Lipid antioxidants: free radical scavenging <i>versus</i> regulation of enzymatic lipid peroxidation
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- Samhan-Arias Alejandro K.
- Center for Free Radical and Antioxidant Health, Department of Environmental and Occupational Health, University of Pittsburgh
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- Tyurina Yulia Y.
- Center for Free Radical and Antioxidant Health, Department of Environmental and Occupational Health, University of Pittsburgh
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- Kagan Valerian E.
- Center for Free Radical and Antioxidant Health, Department of Environmental and Occupational Health, University of Pittsburgh
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The essentiality of polyunsaturated lipids makes membranes susceptible to peroxidative modifications. One of the most contemporary examples includes selective peroxidation of cardiolipin in mitochondria of cells undergoing apoptosis. Cardiolipin peroxidation products are required for the mitochondrial membrane permeabilization, release of pro-apoptotic factors and completion of the cell death program. Therefore, search for effective inhibitors of cardiolipin peroxidation is critical to discovery and development of anti-apoptotic antioxidants. Mitochondria contain significant amounts of α-tocopherol, a well known scavenger of reactive free radicals. In the present study, we used an oxidative lipidomics approach to evaluate the effect of α-tocopherol and its homologues with different lengths of the side-chain such as 2,5,7,8,-tetramethyl-2(4-methylpentyl)-6-chromanol and 2,2,5,7,8-pentamethyl-6-chromanol, on oxidation of tetralinoleoyl cardiolipin induced by cytochrome c in the presence of hydrogen peroxide. Our data indicate that vitamin E homologues inhibit not only accumulation of tetralinoleoyl cardiolipin hydroperoxides but also hydroxy-derivatives of tetralinoleoyl cardiolipin formed in the enzymatic peroxidase half-reaction catalyzed by cytochrome c. This suggests that protective effects of vitamin E homologues against tetralinoleoyl cardiolipin peroxidation catalyzed by cytochrome c/hydrogen peroxide are realized largely due to their effects on the peroxidase activity of cytochrome c towards tetralinoleoyl cardiolipin rather than via their scavenging activity.<br>
収録刊行物
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- Journal of Clinical Biochemistry and Nutrition
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Journal of Clinical Biochemistry and Nutrition 48 (1), 91-95, 2010
一般社団法人 日本酸化ストレス学会
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詳細情報 詳細情報について
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- CRID
- 1390001204673364992
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- NII論文ID
- 130004879239
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- ISSN
- 18805086
- 09120009
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可