Recurrent Micronucleation through Cell Cycle Progression in the Presence of Microtubule Inhibitors

• Nakayama Yuji

  Division of Functional Genomics, Research Center for Bioscience and Technology, Tottori University

• Uno Narumi

  Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University Chromosome Engineering Research Center, Tottori University

• Uno Katsuhiro

  Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University

• Mizoguchi Yamato

  Chromosome Engineering Research Center, Tottori University

• Komoto Shinya

  Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University

• Kazuki Yasuhiro

  Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University Chromosome Engineering Research Center, Tottori University

• Nanba Eiji

  Division of Functional Genomics, Research Center for Bioscience and Technology, Tottori University

• Inoue Toshiaki

  Chromosome Engineering Research Center, Tottori University

• Oshimura Mitsuo

  Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University Chromosome Engineering Research Center, Tottori University

抄録

Although most cell lines undergo mitotic arrest after prolonged exposure to microtubule inhibitors, some cells subsequently exit this state and become tetraploid. Among these cells, limited numbers of rodent cells are known to undergo multinucleation to generate multiple small independent nuclei, or micronuclei by prolonged colcemid treatment. Micronuclei are thought to be formed when cells shift to a pseudo G1 phase, during which the onset of chromosomal decondensation allows individual chromosomes distributed throughout the cell to serve as sites for the reassembly of nuclear membranes. To better define this process, we used long-term live cell imaging to observe micronucleation induced in mouse A9 cells by treating with the microtubule inhibitor colcemid. Our observations confirm that nuclear envelope formation occurs when mitotic-arrested cells shift to a pseudo G1 phase and adopt a tetraploid state, accompanied by chromosome decondensation. Unexpectedly, only a small number of cells containing large micronuclei were formed. We found that tetraploid micronucleated cells proceeded through an additional cell cycle, shifting to a pseudo G1 phase and forming octoploid micronucleated cells that were smaller and more numerous compared with the tetraploid micronucleated cells. Our data suggest that micronucleation occur when cells shift from mitotic arrest to a pseudo G1 phase, and demonstrate that, rather than being a single event, micronucleation is an inducible recurrent process that leads to the formation of progressively smaller and more numerous micronuclei.

収録刊行物

• Cell Structure and Function

  Cell Structure and Function 40 (1), 51-59, 2015

  日本細胞生物学会

キーワード

• Micronucleation
• microtubule inhibitor
• live cell imaging

詳細情報

• CRID: 1390001204694492032
• NII論文ID: 130004885868
• NII書誌ID: AA0060007X
• DOI: 10.1247/csf.14005
• ISSN: 13473700
• ISSN: 03867196
• NDL書誌ID: 027281677
• PubMed: 25736016
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027281677
• Web Site: https://search.jamas.or.jp/link/ui/2017036843
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可