Enhancing osteoblast-affinity of titanium scaffolds for bone engineering by use of ultraviolet light treatment
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- ISHIJIMA Manabu
- Nihon University Graduate School of Dentistry Laboratory of Bone and Implant Sciences (LBIS), The Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry Department of Partial Denture Prosthodontics, Nihon University School of Dentistry
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- SOLTANZADEH Pooya
- Laboratory of Bone and Implant Sciences (LBIS), The Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry
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- HIROTA Makoto
- Laboratory of Bone and Implant Sciences (LBIS), The Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry
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- TSUKIMURA Naoki
- Department of Partial Denture Prosthodontics, Nihon University School of Dentistry
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- SHIGAMI Tomohiko
- Department of Partial Denture Prosthodontics, Nihon University School of Dentistry
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- OGAWA Takahiro
- Laboratory of Bone and Implant Sciences (LBIS), The Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry
書誌事項
- タイトル別名
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- <b>Enhancing osteoblast-affinity of titanium scaffolds for bone engineering by use of ultraviolet light tr</b><b>eatment </b>
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抄録
Ultraviolet (UV) treatment immediately prior to use is attracting attention as an effective surface conditioning method for titanium to improve osteoblast-affinity. The affinity of titanium to osteoblasts in two-dimensional plate culture has been well studied, but that in three-dimensional cultures remains unclear. Here, we examined the effect of UV treatment on titanium scaffolds, comprising micro-thin titanium fibers, used in bone engineering. Titanium scaffolds, with and without UV treatment, were seeded with rat bone marrow derived osteoblasts, and the number of cells attached to scaffolds and osteoblastic phenotype in the cultures were examined. UV treatment improved the wettability of scaffolds and significantly reduced the percentage of surface carbon. Along with these physicochemical changes in the scaffolds, cell attachment increased by a factor of 1.3 as compared to that of the untreated control. In addition, alkaline phosphatase activity and calcium deposition significantly increased by a factor of 2.3 and 2.0, respectively. Robust formation of mineralized structures consisting of clear peaks of calcium and phosphorus was observed in the UV-treated scaffolds. The observed increase in osteoblast affinity and capability of mineralized matrix formation indicates the potential use of UV-treated titanium scaffolds for bone engineering.
収録刊行物
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- Biomedical Research
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Biomedical Research 36 (1), 55-62, 2015
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