Determination of Cyclamate and Dulcin by HPLC and LC/MS/MS and Systematic Analysis of Eight Types of Sweeteners

• MATSUMOTO Hiroko

  Tama Branch Institute, Tokyo Metropolitan Institute of Public Health

• HIRATA Keiko

  Tama Branch Institute, Tokyo Metropolitan Institute of Public Health

• SAKAMAKI Narue

  Tama Branch Institute, Tokyo Metropolitan Institute of Public Health

• HAGINO Kayo

  Tama Branch Institute, Tokyo Metropolitan Institute of Public Health

• USHIYAMA Hirofumi

  Tokyo Metropolitan Institute of Public Health

Bibliographic Information

Other Title

• サイクラミン酸、ズルチンのHPLCによる定量及びLC/MS/MSによる確認と8種甘味料の系統的分析

Abstract

Systematic analysis of 8 artificial sweeteners, namely, saccharin aspartame sucralose acesulfame K neotame cyclamate (CY) dulcin (DU) and alitame, was partially improved. Chopped or homogenized samples were packed into cellulose tubing with 0.01mol/L hydrochloric acid containing 10% sodium chloride, and dialyzed against 0.01mol/L hydrochloric acid for 24-48 hours. The dialyzate was cleaned up using a solid-phase extraction method with an Oasis HLB cartridge for CY and a Sep-Pak Vac C18 cartridge for DU. The average recovery of CY and DU from 9 types of foods spiked with 200μg/g was above 96%. Their detection limits were 5μg/g in samples. Furthermore, CY and DU were both successfully identified by liquid chromatography with tandem mass spectrometry.

Journal

• ChemoBio Integrated Management

  ChemoBio Integrated Management 6 (1), 25-35, 2010

  ChemoBio Integrated Management Society

Keywords

• cyclamate
• artificial sweetener
• HPLC
• LC/MS/MS
• solid-phase extraction

Details

• CRID: 1390282680230852992
• NII Article ID: 130004943405
• DOI: 10.11171/chemobio.6.25
• ISSN: 13499041
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed