Construction of In Vitro Cardiomyocyte Electrical Conduction Line

• Kurotsu Mariko

• Machida Naoki

• Ogawa Emiyu

• Arai Tsunenori

抄録

In order to study condition of arrhythmia treatment by a photosensitization reaction, we developed in vitro cardiomyocyte electrical conduction (EC) line to study EC block by photosensitization reaction. Cardiomyocytes were cultivated on 10 mmφ cover glasses. Cultivated and non-cultivated width were 60 and 300 μm, respectively; for 3-4 days, 37C°, 5% CO₂. Cardiomyocyte cultivate concentration were 2.0-8.0×10⁵ cells/ml. EC of cardiomyocite was measured by 5 μM Fluo-4 AM (Ca²⁺ probe) with a confocal microscope system. Frame rate of EM-CCD was 400 ms. Cadiomyocyte was cultivated in a confluent line by 4.0×10⁵ cells/ml. In 4 days cultivated 4.0×10⁵ cells/ml cardiomyocite line, 1 /s synchronized pulsation was measured as Fluo-4 AM intensity change. Cross correlation coefficient was about 0.8. This result indicated that cardiomyocyte EC was occurred.

収録刊行物

• 生体医工学

  生体医工学 52 (Supplement), O-123-O-124, 2014

  公益社団法人 日本生体医工学会

詳細情報

• CRID: 1390001205267638400
• NII論文ID: 130004948142
• DOI: 10.11239/jsmbe.52.o-123
• ISSN: 18814379
• ISSN: 1347443X
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可