Intracellular Material Injection to Cell Population by Photochemical Local Cell Membrane Perforation

• Inomata Kei

• Ohba Takayoshi

• Mori Takaaki

• Konno Naoto

• Komatu Shingo

• Orito Yusuke

• Unuma Shogo

• Suzuki Kouki

• Ito Keiichi

• Saito Takashi

抄録

We found a unique cell membrane perforation method which is applying photochemical oxidation on a limited area of cell membrane, with high survival rate. We recently developed a photosensitizer included nano needle array perforator for mass cell processing and the robotic processing system. The system had still some instability but in this study, we modified the whole system to improve the mechanical accurancy.The perforator is processed by nanoimprint, with a novel fine mold. The material is silicone polymer including photosensitizer Hematoporphyrin and iron oxide for magnetic connect to the processor. The dimensions of the needle are follows: diameter and hight 1.7μm, pitch 3.0μm. Excitation light is blue bandpass filtered 100W mercury lamp beam. With the system, we could inject membrane impermeable fluorescent dye AlexaFluor594 to rat PC12 cells and Human coronary artery endothelial cells. We will improve the system for various applications, e.g. transgenic process etc.

収録刊行物

• 生体医工学

  生体医工学 52 (Supplement), O-198-O-199, 2014

  公益社団法人 日本生体医工学会

詳細情報

• CRID: 1390001205267507328
• NII論文ID: 130004948164
• DOI: 10.11239/jsmbe.52.o-198
• ISSN: 18814379
• ISSN: 1347443X
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可