Nanoneedle technology to investigate cytoskeletal proteins in living cells

• Nakamura Chikashi

抄録

We developed a method to detect intracellular cytoskeletal proteins using antibody-functionalized nanoneedle. The nanoneedles are ultra-thin cylindrical-shaped AFM probes sharpened to 200 nm in diameter. Following insertion, the nanoneedle was retracted from the live cell and the mechanical force needed to unbind the antibody-target protein complex was measured, we named the peak force “fishing force”. Using this method, intermediate filament proteins, actin filaments and microtubules were successfully detected. The intermediate filament proteins, nestin and neurofilament, which are well-known marker proteins of neural cells could be detected in single living cells, mouse embryonic carcinoma P19, within a minute.

収録刊行物

• 生体医工学

  生体医工学 52 (Supplement), SY-39-SY-40, 2014

  公益社団法人 日本生体医工学会

詳細情報

• CRID: 1390282680243414016
• NII論文ID: 130004948322
• DOI: 10.11239/jsmbe.52.sy-39
• ISSN: 18814379
• ISSN: 1347443X
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可