Internalization of Large Macromolecules in Human Chang Liver Cells by Extracellular ATP
-
- SIT K. H.
- Department of Anatomy
-
- BAY B. H.
- Department of Anatomy
-
- WONG K. P.
- Department of Biochemistry, Faculty of Medicine, National University of Singapore
この論文をさがす
抄録
The home of all intermediary metabolism and the cytoskeleton, viz. the cytosol, appears inaccessible to large macromolecules in the living state without severe perturbations. ATP-induced cytosolic penetration due to poration which allow passage of<1000 mol. wt. particles have been cited. We report here that at similar milli-molar dosage levels of ATP, internalization of 2,000,000 mol. wt dextran particles as well as 0.92 μm and 2.17 μm latex beads could be demonstmted. At ultrastructural level, the internalized beads were seen distributed in 2 sites on the cytoplasm of the cell, viz. (a) inside vacuoles and (b) outside vacuoles, in the cytosol. The vacuolar distribution suggests endocytic entry, while the cytosolic distribution suggests a cellular sorting process where the boundary of the bead is processed or sorted out of the membranerecycling pathway.
収録刊行物
-
- Okajimas Folia Anatomica Japonica
-
Okajimas Folia Anatomica Japonica 70 (6), 247-253, 1994
Editorial Board of Okajimas Folia Anatomica Japonica
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001205460675456
-
- NII論文ID
- 130004990606
-
- COI
- 1:CAS:528:DyaK2cXlsFCitb4%3D
-
- ISSN
- 0030154X
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可