High-Sensitive Analysis of Oligopeptide-Induced Cell Penetration Using Phospholipid Polymer Nanoparticles Containing Quantum Dots

• Goto Yusuke

  Department of Materials Engineering, The University of Tokyo

• Matsuno Ryosuke

  Department of Materials Engineering, The University of Tokyo Center for NanoBio Integration (CNBI), The University of Tokyo

• Konno Tomohiro

  Department of Materials Engineering, The University of Tokyo Center for NanoBio Integration (CNBI), The University of Tokyo

• Takai Madoka

  Department of Materials Engineering, The University of Tokyo Center for NanoBio Integration (CNBI), The University of Tokyo

• Ishihara Kazuhiko

  Department of Materials Engineering, The University of Tokyo Department of Bioengineering, The University of Tokyo Center for NanoBio Integration (CNBI), The University of Tokyo

抄録

We developed new polymer nanoparticles containing quantum dots with artificial cell membrane-biointerface as a highly sensitive bioimaging probe. These nanoparticles were prepared by assembling phospholipid polymer as a platform and oligopeptide as a bioaffinity moiety on the surface of the nanoparticles. They showed high resistance to non-specific cellular uptake from HeLa cells due to the nature of phospholipid polymer with phosphorylcholine groups. On the other hand, when arginine octapeptide was immobilized on their surface, they could permeate the membrane of HeLa cells effectively and good fluorescence based on quantum dots could be observed. Cytotoxicity and inflammation reaction were not produced by these nanoparticles even after immobilization of octapeptide. Thus we obtained stable fluorescent polymer nanoparticles covered with artificial cell membrane, which are useful as an excellent bioimaging probe evaluation for biomolecular function in the target cells.

収録刊行物

• Transactions of the Materials Research Society of Japan

  Transactions of the Materials Research Society of Japan 34 (2), 189-192, 2009

  一般社団法人 日本MRS

キーワード

• polymer nanoparticles
• quantum dots
• phospholipid polymers
• bioimaging
• biomolecular function

詳細情報

• CRID: 1390282680489785216
• NII論文ID: 130005003954
• DOI: 10.14723/tmrsj.34.189
• COI: 1:CAS:528:DC%2BD1MXhtVOms7zO
• ISSN: 21881650
• ISSN: 13823469
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可