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- Fukuda Masatora
- Department of Socio-Enviromental Energy Science, Graduate School of Energy Science, Kyoto University
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- Hayashi Hironori
- Institute of Advanced Energy, Kyoto University
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- Hasegawa Tetsuya
- Institute of Advanced Energy, Kyoto University
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- Morii Takashi
- Institute of Advanced Energy, Kyoto University CREST, JST
抄録
Biologically active amines play major roles in the regulation of movement and are implicated in the pathophysiology of Parkinson’s and Huntington’s diseases, psychosis, and drug addiction. Fluorescent biosensors based on the biological macromolecule receptor are useful tool for investigating the function of biologically active amines. Our strategy of the stepwise molding using a ribonucleopeptide (RNP) framework provides fluorescent biosensors with a variety of binding and optical characteristics for small molecules. Here we report fluorescent RNP sensors for histamine with a variety of binding and signal-transducing characteristics. Combination of RNA subunits of histamine-binding RNP receptors obtained by in vitro selection and a Rev peptide modified with 7-methoxycoumarin-3-carboxylic acid afforded a fluorescent RNP sensor with distinct signaling characteristics in the changes of fluorescence emission intensity upon binding to histamine. The fluorescent histamine sensor showed distinct selectivity for histamine over structurally related histamine analogs, such as imidazole, ethylamine and L-histidine.
収録刊行物
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- Transactions of the Materials Research Society of Japan
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Transactions of the Materials Research Society of Japan 34 (3), 525-527, 2009
一般社団法人 日本MRS
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390282680490358656
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- NII論文ID
- 130005004037
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- COI
- 1:CAS:528:DC%2BD1MXhsFKktbzN
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- ISSN
- 21881650
- 13823469
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可